Facilitation of fatty acid uptake by CD36 in insulin-producing cells reduces fatty-acid-induced insulin secretion and glucose regulation of fatty acid oxidation

Biochimica Et Biophysica Acta
Tina WallinAnneli Björklund

Abstract

Facilitation of fatty acid uptake in beta cells could potentially affect beta cell metabolism and secretory function; however such effects have not been clearly documented. CD36 facilitates uptake of fatty acids (FA) in muscle and adipose tissue and is likely to exert a similar effect in beta cells. We investigated the impact of over-expressing CD36 on fatty acid uptake and beta cell function by a Tet-on system in INS-1 cells. Doxycycline dose-dependently increased the CD36 protein with localization mainly in the cell membrane. Over-expression increased both specific uptake and efflux of oleate whereas intracellular glycerides were only marginally increased and incorporation of 14C-oleate or -palmitate into di- or triglycerides not affected. The normal potentiation of glucose-induced insulin secretion by acute addition of FA (50-100 micromol/l oleate and palmitate) was lost and the normal inhibitory effect of high glucose both on oleate oxidation and on the activity of carnitine palmitoyltransferase I was reduced. Over-expression did not induce apoptosis. We conclude that induction of the CD36 transporter increases uptake of FA, the consequences of which are blunting of the functional interplay between glucose and FA on insulin...Continue Reading

Citations

Aug 25, 2015·Biochimica Et Biophysica Acta·Udayakumar KarunakaranKyu Chang Won
Mar 9, 2010·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Noel G Morgan, Shalinee Dhayal
Mar 21, 2012·Biochemical and Biophysical Research Communications·Yong-Woon KimKyu Chang Won
Jun 18, 2011·Biochemical and Biophysical Research Communications·Louise T DalgaardJens H Nielsen
Sep 25, 2016·Journal of Diabetes and Its Complications·Jun Sung MoonKyu Chang Won
Feb 6, 2017·Experimental & Molecular Medicine·Luong Dai LyKyu-Sang Park
Mar 23, 2018·Nutrients·Paloma Acosta-Montaño, Víctor García-González
Apr 7, 2016·Journal of Korean Medical Science·Ji Sung YoonHyoung Woo Lee
Feb 18, 2014·The Journal of Clinical Endocrinology and Metabolism·Henri HonkaPirjo Nuutila
Oct 25, 2011·Food & Function·Almudena OrtegaFrancisco J G Muriana
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Mar 28, 2019·Journal of Molecular Endocrinology·Z MaA Björklund
Apr 30, 2020·Diabetes & Metabolism Journal·Jun Sung MoonKyu Chang Won
Dec 22, 2020·Frontiers in Cell and Developmental Biology·Xin WangDong Zhao
Apr 13, 2021·Molecular and Cellular Biochemistry·Monica Losada-Barragán

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