Facilitation of L-type calcium current in thalamic neurons

Journal of Neurophysiology
P J Kammermeier, S W Jones

Abstract

We have studied facilitation of the L-type calcium current in neurons acutely isolated from the ventrobasal nucleus of the rat thalamus. Currents were recorded after pretreatment with 1 microM omega-conotoxin GVIA and 5 microM omega-conotoxin MVIIC, to better isolate L-current. Long, strong depolarizations induced slow tail currents at negative voltages, but did not affect currents at voltages where channels were strongly activated. The initial peak tail current was not measurably increased. The time course of recovery from facilitation paralleled the time course of the tail current, indicating that facilitation does not outlast channel closing. The kinase inhibitors staurosporine and H-7 and the phosphatase inhibitor okadaic acid had no significant effect on L-current facilitation compared with control, but facilitation was greater with H-7 than with okadaic acid. The guanosine 5'-triphosphate (GTP) analogs GTP-gamma-S and GDP-beta-S did not affect facilitation. We conclude that L-current facilitation in thalamic neurons does not result from Ser/Thr phosphorylation, although phosphorylation may modulate facilitation. This form of facilitation differs kinetically and pharmacologically from facilitation induced by activation of ...Continue Reading

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Citations

Jun 2, 2006·Journal of Neurophysiology·Timm SchubertAndreas Feigenspan
Apr 13, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yisheng LuZuoping Xie
Mar 29, 2012·The Bulletin of Tokyo Dental College·Takayuki EndohKenji Sueishi
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May 3, 2000·The European Journal of Neuroscience·K P CarlinR M Brownstone
Jan 11, 2003·Journal of Neurophysiology·Randall K Powers, Marc D Binder
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Sep 3, 2013·Cell Calcium·Roman V Frolov, Satpal Singh

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