Facilitation of proteasomal degradation of p27Kip1 by N-terminal cleavage and their sequence requirements

FEBS Letters
Katsuya HiranoHideo Kanaide

Abstract

The sequence requirement for N-terminal cleavage and the proteasomal degradation of p27Kip1 and their relationship was investigated. Residues 5-8 were required for the cleavage and the mutation of S10 to E inhibited the cleavage. The C-terminal PEST sequence was necessary for the degradation and residue R165 was found to play an important role in the degradation. The inhibition of the cleavage by deleting residues 5-8 inhibited the degradation, while the fragment mimicking the cleavage product accelerated the degradation. Both the cleavage and degradation demonstrated a similar sensitivity toward proteasome inhibitors and ATP depletion. These two processes are thus suggested to be tightly linked and sequential.

References

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Citations

Dec 2, 2009·The Journal of Comparative Neurology·Raquel Martín-IbáñezJosep M Canals
Oct 22, 2005·Proteomics·Véronique MalardEric Quéméneur

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