FACS technology used in a new rapid bacterial detection method

Transfusion Medicine
M SchmidtE Seifried

Abstract

Culture methods for bacterial detection (BacT/ALERT or Pall eBDS) are currently implemented in blood donor screening procedures in many countries. Experience in the first years after implementation of these detection assays showed that although the analytical sensitivity was extremely high (about 1 CFU mL(-1)), the majority of these platelets were still transfused before a positive screening result was attained. Rapid technologies were developed to more effectively prevent transfusion-transmitted bacterial infection. In this study, a new rapid bacterial detection method based on fluorescence-activated cell sorting (FACS) technology was developed. Bacteria were stained with thiazole orange dye for 5 min and measurement was taken immediately after staining. The entire process took only 30 min. Six transfusion-relevant bacteria strains were tested in a spiking study. Without pre-incubation in a special bacteria growth medium, analytical sensitivity ranged between 10(5) CFU mL(-1) (Klebsiella oxytoca and Serratia marcesens) and 10(3) CFU mL(-1) (Escherichia coli). Sensitivity could be improved to 10(1) CFU mL(-1) for all tested bacteria by adding a pre-incubation step (6 h at 37 degrees C). Although preliminary in nature, results o...Continue Reading

References

Dec 1, 2001·Seminars in Hematology·Morris A Blajchman, M Goldman
Jan 18, 2005·Clinical Microbiology Reviews·Mark E Brecher, Shauna N Hay
Apr 12, 2005·Transfusion·P A W te BoekhorstD J van Rhenen
Jan 10, 2006·Transfusion·Harald MohrT H Müller
Jan 31, 2006·Transfusion·Paul J Schmidt, Paul M Ness

Citations

Oct 22, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·M SchmidtE Seifried
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Oliver KaroT Montag
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Mariam Klouche, Uwe Schröder
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·T Montag
Dec 22, 2006·Vox Sanguinis·M SchmidtGabriele Walther-Wenke
Oct 28, 2016·Journal of Microbiological Methods·Célia FontanaJean Reyes
Nov 8, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irene O L McHugh, Ayly Ling Tucker

Related Concepts

Bacteriological Techniques
Blood Platelets
Flow Cytometry
Streak Plate Count
Platelet Transfusion
Tissue and Organ Selection

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