Factor derived from human lung carcinoma associated with hypercalcemia mimics the effects of parathyroid hormone on phosphate transport in cultured renal epithelia

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
L PizurkiJ P Bonjour

Abstract

A decrease in renal tubular reabsorption of inorganic phosphate (Pi) can be observed in hypercalcemia of malignancy. In the present study we investigated the effect of serum-free conditioned medium (CM) from cells, derived from a lung carcinoma (BEN) of a hypercalcemic patient, and of PTH on cyclic AMP (cAMP) production and sodium-dependent Pi transport (NaPiT) in epithelia of two renal cell lines. In opossum kidney cells (OK), PTH is known to enhance cAMP production and inhibit NaPiT; in contrast, in LLC-PK1 cells, PTH has no effect on NaPiT since this kidney cell line is devoid of PTH receptors. In OK cells, BEN CM induced a three- to fourfold increase of cAMP production, which was blunted by the PTH inhibitors bPTH(3-34) and bPTH(7-34). NaPiT, as assessed by measuring the initial rate of Pi uptake, was inhibited in a dose-dependent manner by BEN CM, with an effect maximal between 1h30 and 6 hr of incubation (40 +/- 4% and 47 +/- 4%, respectively), corresponding to the effect produced by 1-3 nM bPTH(1-34). The Na-dependent transport of a glucose analog was affected neither by BEN CM nor by PTH. In LLC-PK1 cells, neither BEN CM nor PTH altered cAMP production nor NaPiT after 1h30 of incubation. At 6 hr, BEN CM caused a slight ...Continue Reading

References

Jul 28, 1978·Biochemical and Biophysical Research Communications·S R GoldringS M Krane
Mar 1, 1975·Acta Endocrinologica·H MinneR Ziegler
Sep 1, 1975·British Journal of Cancer·M EllisonA M Neville
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J M MoseleyJ D Zajac
Jul 1, 1986·Endocrinology·G J StrewlerR A Nissenson
Jul 1, 1986·The American Journal of Physiology·K Malmström, H Murer
Nov 19, 1986·Biochimica Et Biophysica Acta·S MohanD J Baylink
Oct 1, 1972·The Journal of Clinical Endocrinology and Metabolism·G C SchusslerT Nemoto
May 1, 1971·Endocrinology·B F RiceM C Miller
Jul 1, 1980·The Journal of Clinical Endocrinology and Metabolism·S C KukrejaP A Johnson
Apr 1, 1981·The Journal of Clinical Endocrinology and Metabolism·R K RudeF R Singer
Jun 28, 1984·The New England Journal of Medicine·G R MundyT J Martin
Jan 1, 1984·Reviews of Physiology, Biochemistry and Pharmacology·J P Bonjour, J Caverzasio
Dec 1, 1984·Endocrinology·P J GkonosA F Stewart
Aug 11, 1983·The New England Journal of Medicine·E L SimpsonJ W Jacobs
May 1, 1983·Calcified Tissue International·D A SicaG R Mundy

❮ Previous
Next ❯

Citations

Feb 19, 2004·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Rafik A AbdelsayedNabil A Ibrahim
Dec 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·F LawJ P Bonjour

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.