Factor H in porcine seminal plasma protects sperm against complement attack in genital tracts.

The Journal of Biological Chemistry
Tomohisa SakaueI Ohkubo

Abstract

We found that factor H (FH) exists in porcine seminal plasma. Purified FH strongly inhibited serum alternative pathway complement activation against lipopolysaccharide. The molecular weight, pI, and heparin-binding activity of the purified protein were different from those of purified FH from porcine serum. The complement regulatory activity of seminal plasma FH was approximately 2-fold stronger than that of serum FH. Treatment of purified serum FH with sialidase and N-glycosidase F gave almost the same results as those of seminal plasma FH. The deletion of sialic acid from the carbohydrate chains of both FHs contributed to heparin-binding and complement regulatory activities. Results of reverse transcriptase-PCR, Western blot analysis, and immunohistochemistry showed that seminal plasma FH is mainly secreted from epithelial cells of the seminal vesicle in male genital tracts. FH was also detected in the outer acrosomal region of ejaculated sperm by immunofluorescence staining, and found that the purified FH from the sperm membrane has the same complement regulatory activity as that of seminal plasma FH. The ejaculated sperm possessing FH in the outer acrosomal region considerably evaded complement attack. We also found that th...Continue Reading

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Citations

Jun 28, 2012·BMC Veterinary Research·Isabel Moreno-IndiasRobert B Sim
Jul 19, 2014·Theriogenology·Verónica González-CadavidArlindo A Moura
Jun 16, 2012·Biochemical and Biophysical Research Communications·Li-Ping AnHisakazu Ogita
Apr 26, 2016·Journal of Proteomics·Cristina Perez-PatiñoJordi Roca
Jul 28, 2016·Journal of Ovarian Research·Rebeca López-ÚbedaSebastian Canovas
Oct 31, 2019·Molecular Biology and Evolution·Melissah RoweSteve Dorus
Feb 23, 2020·Molecular Reproduction and Development·Gry Brandt Boe-HansenMichael McGowan
Jan 4, 2021·Tropical Animal Health and Production·Paula Lorena SoutoAlexandre Floriano Ramos
Jun 25, 2016·The Journal of Biological Chemistry·Farhad KhosraviAndreas Meinhardt
Feb 24, 2020·Theriogenology·Jordi RocaInmaculada Parrilla

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