Factor VIII influences binding of factor IX and factor X to intact human platelets

Thrombosis Research
W Muntean, B Leschnik

Abstract

To investigate the influence of factor VIII on the binding of factors IX and X to the surface of intact human platelets, washed collagen-stimulated platelets were incubated with factor IX and factor X in the presence or absence of factor VIII. Platelets were then lysed and IX:Ag and X:Ag were determined in the platelet lysate. Platelet bound IX:Ag was higher after incubation of platelets with factor IX in the presence of native or activated factor VIII than after incubation of platelets with factor IX alone. Thrombin degraded factor VIII did not support binding of factor IX to stimulated platelets. Preactivation of factor IX enhanced binding to platelets; influence by factor VIII on binding to platelets was greater with native factor IX than with preactivated factor IX. Presence of native or activated factor VIII also increased binding of factor X to platelets. In contrast to factor IX, preactivation of factor X did not influence binding to platelets, but in the presence of factor VIII preactivation of factor X increased binding of factor X to platelets. Our data suggest that mediation of binding of factors IX and X to the surface of platelets is one of the mechanisms by that factor VIII exerts its cofactor function in the acti...Continue Reading

References

Oct 1, 1977·The Journal of Clinical Investigation·M E Switzer, P A McKee
Dec 7, 1983·Biochimica Et Biophysica Acta·E M BeversR F Zwaal
Dec 1, 1984·European Journal of Clinical Investigation·W Muntean, H J Rothwangl
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Jan 1, 1981·Annals of the New York Academy of Sciences·K G MannP B Tracy

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Citations

Oct 1, 2013·Expert Opinion on Therapeutic Targets·Silvia Montoro-GarcíaGregory Y H Lip
Dec 20, 2007·Journal of Thrombosis and Haemostasis : JTH·B I ErikssonUNKNOWN Fixit Study Group
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·S S AhmadP N Walsh
Aug 1, 2007·Journal of Thrombosis and Haemostasis : JTH·P E BockI M A Verhamme
Jul 14, 2011·Cardiovascular Therapeutics·Christopher Roser-JonesRichard C Becker
Oct 30, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Karin LeidermanAaron L Fogelson

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