Factors accounting for perinatal occurrence of pulsatile gonadotropin-releasing hormone secretion in vitro in rats

Biology of Reproduction
Anne-Simone ParentJean-Pierre Bourguignon

Abstract

Our aim was to study the inhibitory and facilitatory factors possibly accounting for the undetectable activity of the GnRH pulse generator in late fetal life in vitro and its awakening in early postnatal life. Gamma aminobutyric acid (GABA(A)) receptor antagonism using SR 95 531 did not cause any secretory pulse in fetal explants, whereas a significant stimulation of GnRH pulse frequency was obtained at 5 and 15 days. GnRH secretory response to repeated N-methyl-D-aspartate (NMDA) stimulation showed progressive disappearance, indicating that the inhibitory autofeedback was operating. GnRH release caused by glutamine was respectively 9% and 20% of that evoked by glutamate in fetal and 5-day-old rats whereas both amino acids were equally active at 15 days. Explants obtained after cesarean section performed at onset of labor did not show any secretory pulse, while pulses could be observed with explants obtained 2 h after vaginal delivery. Incubation of fetal explants with oxytocin (10(-8) M) or prostaglandin E2 (PGE2) (10(-6) M) resulted in occurrence of GnRH secretory pulses. A facilitatory effect of the oxytocin was shown to persist on Days 1, 5, and 15 and inhibitory effects of an oxytocin receptor antagonist provided some evid...Continue Reading

References

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Citations

Nov 23, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Grégory RasierJean-Pierre Bourguignon
Nov 6, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michael J WollerDavid H Abbott
Nov 28, 2007·Endocrinology·Anne-Simone ParentJean-Pierre Bourguignon
Mar 12, 2016·Journal of Pediatric Gastroenterology and Nutrition·Anni LarnkjærKim F Michaelsen

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Methods Mentioned

BETA
cesarean section

Software Mentioned

Pulsar

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