PMID: 9450790Feb 5, 1998Paper

Factors affecting cardiolipin antibody assays: modification with polyethylene glycol compound

British Journal of Haematology
D C Kilpatrick

Abstract

Anti-cardiolipin antibody (aCL) measurement is only semi-reproducible, and current assays detect irrelevant as well as clinically significant antibodies. Factors found to influence results included the source of the enzyme-linked immunoabsorbent assay (ELISA) plate, and its pretreatment with solvents; the nature of the blocking solution; and the composition of the diluent used for reagents. Fetal calf serum (FCS) in the diluent appeared to reduce nonspecific (clinically irrelevant) binding and was not simply a source of beta2-glycoprotein-1 (beta2GP1). A polyethylene glycol compound was found to be an effective blocking agent, and its use enhanced the discrimination between positive and negative samples. A high level of variation may be inherent in aCL-ELISA methodology, but the use of polyethylene glycol compound appeared to be a helpful modification.

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Citations

May 1, 2002·Journal of Clinical Microbiology·Yevgeniy Y StudentsovRobert D Burk
Jun 26, 2012·Journal of Nanobiotechnology·Junkun HeJeffrey T Mason
Jan 22, 2009·Journal of Neuroinflammation·Lawrence L HorstmanAlireza Minagar
Oct 16, 2008·Journal of Immunological Methods·Anna Ketelsen, Håkan Eriksson
Apr 25, 2007·Critical Reviews in Clinical Laboratory Sciences·P G VlachoyiannopoulosP Tsiligros
May 10, 2007·Clinical and Experimental Immunology·R RollaG Bellomo
Oct 28, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Olga A CherepanovaGary K Owens
Feb 15, 2002·BJOG : an International Journal of Obstetrics and Gynaecology·Eric VerspyckJeanne Yvonne Borg

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