Abstract
Biological factors are known to influence the fetal fraction (FF) of cell-free DNA and may also influence the accuracy of non-invasive prenatal testing. NIPT from 5267 mixed risk women across three specialist clinics in Australia were analyzed. Multivariable regression analysis was used to determine whether maternal characteristics, ultrasound, and placental biomarkers affect FF and test accuracy. FF ranged from 4% to 37% (mean 11.6%). Body mass index (BMI), gestation, and placental biomarkers were found to be significant factors associated with FF. For each unit increase in BMI, the logarithmically transformed FF, (lnFF), mean value decreased by 0.027. Each week increases in gestation, lnFF increased by 0.023. Each unit increase in free BhCG, PAPPA, and PlGF, the lnFF increased by 0.065, 0.050, and 0.17, respectively. There was no significant association between FF with either maternal age or nuchal translucency. The false-positive cases and one false-negative case did not have lower FF than the true-positive cases. The fetal fraction in maternal plasma cfDNA increased with gestational age, serum pregnancy-associated plasma protein A (PAPP-A), β-hCG, and PlGF and decreased with increasing maternal BMI. There was no significant...Continue Reading
References
Jul 7, 2001·Placenta·M MetzenbauerK Philipp
Aug 16, 2008·Clinical Chemistry·Fiona M F LunY M Dennis Lo
Jul 2, 2009·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Fergus ScottAndrew McLennan
Jan 7, 2010·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·T KasdaglisA A Baschat
Sep 15, 2010·Clinical Chemistry·Y M Dennis Lo
Feb 12, 2011·American Journal of Obstetrics and Gynecology·Mathias EhrichDirk van den Boom
Apr 27, 2011·Clinical Chemistry·Amy J SehnertRichard P Rava
Oct 19, 2011·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Glenn E PalomakiJacob A Canick
Jan 31, 2012·Fetal Diagnosis and Therapy·Pranav PandyaKypros H Nicolaides
May 11, 2012·Fetal Diagnosis and Therapy·Ghalia AshoorKypros H Nicolaides
Oct 31, 2012·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·G AshoorK H Nicolaides
Nov 22, 2012·Obstetrics and Gynecology·UNKNOWN American College of Obstetricians and Gynecologists Committee on Genetics
Dec 5, 2012·PloS One·Jeanne SibiudeVassilis Tsatsaris
Mar 8, 2013·Fetal Diagnosis and Therapy·L C Y PoonK H Nicolaides
Apr 6, 2013·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Anthony R GreggM S Watson
Apr 18, 2013·Prenatal Diagnosis·Amin R MazloomCosmin Deciu
Aug 10, 2013·Prenatal Diagnosis·Yanlin WangWeiwei Cheng
Sep 21, 2013·Clinical Chemistry·Richard P RavaDiana W Bianchi
Dec 18, 2013·Fetal Diagnosis and Therapy·Kypros H NicolaidesM M Gil
Jun 5, 2014·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·L J SalomonUNKNOWN ISUOG Clinical Standards Committee
Jul 9, 2014·Obstetrics and Gynecology·Eugene PergamentMatthew Rabinowitz
Sep 25, 2014·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·M S QuezadaK H Nicolaides
Nov 2, 2014·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·T Takoudes, B Hamar
Nov 14, 2014·Prenatal Diagnosis·Jan M M Van LithDiana W Bianchi
Jan 20, 2015·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·H ZhangW Wang
May 28, 2015·Prenatal Diagnosis·Sarah L KinningsCosmin Deciu
Aug 20, 2015·Obstetrics and Gynecology
Jan 29, 2016·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Andrew McLennanFergus Scott
Citations
Feb 21, 2018·Human Reproduction·Timothy J LeeFabricio da Silva Costa
Jul 12, 2018·Obstetrics and Gynecology·Daniel L RolnikFabricio da Silva Costa
Jan 11, 2018·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·D L RolnikA C McLennan
Nov 21, 2018·Reproductive Sciences·Mohak MhatreAndrea Edlow
Dec 11, 2019·Prenatal Diagnosis·Lisa Hui, Diana W Bianchi
Mar 22, 2020·Molecular Genetics & Genomic Medicine·Wei LiZhiyu Peng
Nov 2, 2019·Journal of Medicine and Life·Ioan Dumitru SuciuLucian Pop
Nov 27, 2019·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Ioan SuciuOana Toader
Jul 17, 2020·Prenatal Diagnosis·Rhiannon SandowAndrew C McLennan
Dec 4, 2019·Clinical Case Reports·Kathleen BoneJulie Lauzon
May 31, 2018·AJOB Empirical Bioethics·Hazar HaidarVardit Ravitsky
May 27, 2020·Prenatal Diagnosis·Tachjaree PanchaleeArthur L Beaudet
Jun 20, 2019·F1000Research·Ondrej PösTomáš Szemes
Nov 6, 2020·Archives of Gynecology and Obstetrics·Heike BorthBernd Eiben
Jan 15, 2021·Scientific Reports·Dong ZhaoQiang Xia
Aug 3, 2020·Best Practice & Research. Clinical Obstetrics & Gynaecology·Dana Kimelman, Mary Ellen Pavone
Jan 26, 2021·Health Psychology Open·Valentina Di MatteiMassimo Candiani
Oct 9, 2019·Gynécologie, Obstétrique, Fertilité & Sénologie·C DuvillierF Vialard
May 12, 2021·Prenatal Diagnosis·Liesbeth VossaertIgnatia B Van den Veyver
Aug 17, 2021·Cancer Biology & Therapy·David HsiehchenMuhammad S Beg
Sep 18, 2021·Archives of Gynecology and Obstetrics·Anahita BajkaTilo Burkhardt
Nov 2, 2021·Science Progress·Liang HuFengxiang Wei
Sep 11, 2018·Fetal Diagnosis and Therapy·Sofie KruckowRipudaman Singh
Dec 2, 2021·The Journal of Applied Laboratory Medicine·Henry A ErlichCassandra D Calloway
Dec 15, 2021·Analytical Chemistry·Lucien JackyAditya Rajagopal