Factors affecting expression of the Escherichia coli pilus K99.

Infection and Immunity
R E Isaacson

Abstract

An enzyme-linked antibody centrifuge assay for the detection of Escherichia coli pilus K99 was developed and shown to be a specific and quantitative assay for the detection of cell-bound K99. The data presented demonstrate the usefulness of the assay as a diagnostic tool. Using the assay, several factors that affect expression of K99 were investigated. Expression of K99 was dependent upon the degree of aeration provided: nonaerated bacteria produced little or no K99, whereas aerated bacteria produced large amounts of K99. K99 also appeared to be produced only by logarithmically growing cells, whereas there was a demonstrable decline in the amount of K99 per cell during stationary phase. Glucose was shown to repress K99 expression. At 0.5% glucose, K99 expression was highly repressed. Glucose-mediated repression could be overcome by the addition of cyclic adenosine 3',5'-monophosphate. Several other carbon sources also inhibited K99 expression, including pyruvate, arabinose, and lactose; glycerol was stimulatory.

References

Sep 1, 1977·Infection and Immunity·J F Alderete, D C Robertson
Oct 1, 1975·Infection and Immunity·A Hohmann, M R Wilson
May 1, 1970·Journal of Medical Microbiology·J B Arbuckle

❮ Previous
Next ❯

Citations

Apr 1, 1991·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·C D MullaneyJ A Willgohs
Nov 1, 1993·Molecular Microbiology·O J InoueR E Isaacson
Jan 1, 1988·Antonie van Leeuwenhoek·F K De Graaf
Jan 1, 1983·Critical Reviews in Microbiology·G W Jones, R E Isaacson
Jan 1, 1985·Journal of Dairy Science·S D Acres
Jul 31, 1998·Nutrition·J Alverdy, E Stern
Jun 1, 1982·Microbiological Reviews·W Gaastra, F K de Graaf
Feb 1, 1981·Journal of Clinical Microbiology·C J SmythI Orskov
Oct 14, 2016·EcoSal Plus·J Daniel DubreuilDieter M Schifferli
May 1, 1983·Infection and Immunity·R E Isaacson
Sep 1, 1983·Infection and Immunity·D H FrancisJ D Fritzemeier
Sep 1, 1982·Journal of Bacteriology·B I Eisenstein, D C Dodd

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.