Factors affecting gene expression and activity of heme- and manganese-dependent catalases in Lactobacillus casei strains

International Journal of Food Microbiology
A RicciardiT Zotta

Abstract

Catalases reduce oxidative stress by degrading hydrogen peroxide to molecular oxygen and water. The presence of heme-dependent or manganese-dependent catalases was observed for a long time in lactic acid bacteria (LAB) but, to date, knowledge on the factors affecting gene expression and enzymatic functionality are limited to a very few strains. In this study, the effect of atmosphere of incubation (not aerated static growth vs aerated shaken growth) and supplementation with Fe2+, hemin, Mn2+ or their combinations on the catalase production of respiration-competent strain Lactobacillus casei N87 was evaluated using a 24 factorial design. Kinetics of growth, enzymatic activity, tolerance of oxidative stress and expression of heme- and Mn-catalase genes were assessed. A phylogenetic analysis of heme- and Mn-catalase sequences retrieved for all published LAB genomes was performed. The presence of cofactors, especially when combined, improved biomass production in L. casei N87 in both aerated and not aerated conditions. The genome of L. casei N87 harboured sequences for both catalases and hemin and Mn supplementation was crucial for gene expression and enzyme functionality. Iron and oxygen had an additive stimulatory effect. Toleran...Continue Reading

Citations

Sep 13, 2019·Scientific Reports·Tao YuTingting Guo
Feb 24, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Vanessa Moraes Ramalho CastroRosa Helena Luchese
Oct 9, 2021·Biochemical Society Transactions·Huong Thanh NguyenKwanwoo Shin

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