PMID: 9438033Nov 1, 1991Paper

Factors affecting the determination of total mercury in biological samples by continuous-flow cold vapor atomic absorption spectrophotometry

Biological Trace Element Research
A M Scheuhammer, D Bond

Abstract

The acidic reduction of Hg using a continuous-flow analytical system was evaluated. With 25% SnCl2 as the reductant, characteristic concentrations (sensitivities) of 0.44 microgram/L (open cell) and 0.29 microgram/L (flow-through cell) were obtained using inorganic Hg2+ standards in 1.5% HCl. When CH3Hg+ standards were used, absorption signals were an order of magnitude lower, indicating that Sn(II) is incapable of producing Hg degree from organic Hg in this acidic reduction system. Addition of CdCl2 to the SnCl2 reductant, as suggested by Magos (1) for the reduction of organomercurials under alkaline conditions, was without beneficial effect. Similarly, combining Sn with another reducing agent (hydroxylamine hydrochloride), or a strong alkaline solution (40% NaOH), in the reaction coil of the flow-through system did not significantly enhance the Hg absorption signal for either inorganic or organic Hg. Recovery of Hg from spiked liver homogenates digested at 70-80 degrees C using a HNO3/H2SO4/HCl procedure and stabilized with 0.5 mM K2Cr2O7 was > 85% using either inorganic Hg2+ or CH3Hg+, indicating that this digestion procedure successfully breaks the C-Hg bond to form readily reducible Hg species. Using L-cysteine to stabiliz...Continue Reading

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Citations

Jan 1, 1996·Pathology Oncology Research : POR·László KozmaSzabolcs Gomba
Apr 1, 1994·Biological Trace Element Research·S K AggarwalD A Herold
Jun 4, 2005·Ecotoxicology·Neil M BurgessJoseph D Kaplan
Nov 27, 2007·Ecotoxicology·Neil M Burgess, Michael W Meyer
Apr 1, 1997·Environmental Health Perspectives·M HarnlyL Goldman
Jan 28, 2012·Environment International·Mélanie F GuiguenoJohn E Elliott
Jul 22, 2015·Archives of Environmental Contamination and Toxicology·John E ElliottPatrick Shaw
Jul 6, 2004·Environmental Pollution·S A WeechK M Cheng
Feb 26, 2008·Environmental Pollution·A L M EthierD E Bond
Jul 7, 2007·Journal of Animal Physiology and Animal Nutrition·K LybergJ E Lindberg
Jul 1, 1997·Social Psychiatry and Psychiatric Epidemiology·D W Chan
Jan 1, 2003·European Journal of Oral Sciences·Serap Akyüz, Esber Caglar

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