PMID: 2491613Jan 1, 1989Paper

Factors affecting the insulin autoantibody ELISA

Autoimmunity
L J NellJ W Thomas

Abstract

IgG antibodies to insulin are present in insulin-treated patients and are detected in the prodrome of untreated type I diabetes. Sporadic reports of autoantibodies to insulin suggest that they are also present in other disorders. To establish the incidence of insulin autoantibodies in other endocrine and autoimmune diseases an ELISA was used to examine sera from 529 subjects with no prior insulin therapy. These untreated patients included: normal controls (adults and children), newly-diagnosed type I diabetes, first-degree relatives of diabetics, type II diabetes, Graves' hyperthyroidism, and systemic lupus erythematosus. As a positive control group, 280 insulin-treated patients were studied. Measurement of IgG antibodies by direct binding to insulin coated plates was complicated by differences between adult and pediatric populations and by overlap of binding between treated and untreated subjects. Competitive inhibition with excess soluble human insulin overcame these problems and permitted identification of insulin specific binding. Using this approach insulin antibodies were most frequent in insulin-treated diabetics (98%) and in type I diabetics (37%) prior to treatment. The absolute numbers of subjects with insulin autoant...Continue Reading

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Citations

Nov 5, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Jeanne M SoosDanuta J Herzyk
Mar 29, 2011·Rheumatology International·Jozélio Freire de Carvalho, Maria Teresa Correia Caleiro
May 1, 1990·The American Journal of the Medical Sciences·J A EllerhorstL J Nell
Jul 6, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lucrezia ColonnaRaphael Clynes

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