Factors controlling bacterial attachment and biofilm formation on medium-chain-length polyhydroxyalkanoates (mcl-PHAs)

Colloids and Surfaces. B, Biointerfaces
L MauclaireManfred Zinn

Abstract

Polyhydroxyalkanoate (PHA) is a versatile class of biodegradable and biocompatible biopolyesters accumulated by many bacteria as intracellular carbon storage compound. The largest subclass consists of medium-chain-length (mcl) PHA which has a large potential in medical applications where flexible or elastomeric materials are required. Different extraction and purification methods for mcl-PHA are known to result in variations of polymer purities. In this study it was assessed whether this difference in quality may increase risk of failure of the implant material by enhanced colonization by Gram-negative and Gram-positive model pathogens (Staphylococcus aureus and Escherichia coli curli). Two types of mcl-PHA copolymers, poly(3-hydroxyoctanoate) (PHO) and the less known poly(3-hydroxyundecanoate) (PHUA), were evaluated for an impact of monomeric unit composition and degree of polymer purity on colonization by the model pathogens. It was found that film formation by bacteria on purified PHO and PHUA coatings was lower or similar to the one observed for polystyrene controls. The presence of proteins and lipopolysaccharide impurities originating from biotechnological production contributed to an increase in biofilm development proba...Continue Reading

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Citations

Feb 1, 2011·Applied Microbiology and Biotechnology·Kumar SudeshYan-Fen Lee
Oct 10, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Matthew A GetzlafAndre J van Wijnen
Nov 12, 2013·Biochemical and Biophysical Research Communications·Jieun KimKyung-Jin Kim
Aug 26, 2014·Biochemical and Biophysical Research Communications·Eun-Jung Kim, Kyung-Jin Kim
Jan 3, 2012·International Journal of Biological Macromolecules·Moon Gyu ChungYoung Ha Rhee
Apr 4, 2013·The Journal of Biological Chemistry·Glenn J RapsinskiÇagla Tükel
Nov 6, 2017·International Journal of Biological Macromolecules·Benjamin Antwi PeprahBruce A Ramsay
Aug 10, 2015·ACS Biomaterials Science & Engineering·Judith BöhmlerLydie Ploux

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