Factors influencing fetal macrophage development: I. Reactions of the tumor necrosis factor-alpha cascade and their inhibitors

The Anatomical Record
S P SorokinN A McNelly

Abstract

When fetal rat lungs are explanted to organ culture, precursor angular cells soon convert to nascent macrophages that multiply rapidly as they mature into efficient phagocytes. The present study examines the influence of proinflammatory early cytokines of the tumor necrosis factor-alpha (TNF alpha) cascade on this initial expression of the macrophage phenotype. Fourteen- and 15-day fetal rat lungs were grown for varying periods on an agar-solidified medium with and without test factors added singly or in combination. Growth of the macrophage population was followed daily by light microscopy and quantified by measuring the area of coronas formed as cells emerged from explants. TNF alpha interleukin-1 beta (IL-1 beta) stimulated growth of the macrophage population, as had macrophage- and granulocyte-macrophage colony-stimulating factors (M- and GM-CSFs) in prior studies. Inhibition was obtained by exposure to IL-1 receptor antagonist and antibodies neutralizing the CSFs. Only the effects of TNF alpha were sufficiently delayed to discount possible influence on conversion and growth of nascent macrophages. Two transcription blockers, dexamethasone and pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor NF-kappa B, bo...Continue Reading

References

May 1, 1992·The Journal of Experimental Medicine·R SchreckP A Baeuerle
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B P GiroirB Beutler
Jul 1, 1991·American Journal of Obstetrics and Gynecology·W H KuttehB R Carr
Jan 1, 1991·Annual Review of Immunology·J J OppenheimK Matsushima
Oct 11, 1991·Molecular and Cellular Biology·A S BaldwinL R Coodly
May 1, 1988·The Journal of Clinical Investigation·F HerrmannR H Mertelsmann
Jun 1, 1986·The Journal of Experimental Medicine·C A DinarelloJ V O'Connor
Oct 16, 1986·Nature·K W Miller
Oct 1, 1995·Journal of Leukocyte Biology·P Roth, E R Stanley
Aug 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T A Tran-ThiP A Baeuerle
Jan 1, 1995·Anatomy and Embryology·M A Wride, E J Sanders
Jan 1, 1995·Advances in Immunology·E B Kopp, S Ghosh
Oct 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T JaskollM Melnick
Jan 1, 1994·Annual Review of Cell Biology·P van der GeerR A Lindberg
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Nov 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M A Wride, E J Sanders
Jul 1, 1993·Fetal Diagnosis and Therapy·A AbbasK H Nicolaides
Aug 1, 1993·American Journal of Obstetrics and Gynecology·S L OpsjłnR Austgulen
Jan 1, 1993·Advances in Immunology·S AkiraT Kishimoto
Dec 1, 1995·Immunology Today·S L KunkelJ Westwick

❮ Previous
Next ❯

Citations

Oct 23, 2015·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Tao LiuZi-Jiang Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.