Factors influencing fetal macrophage development: II. Effects of the PDGF subfamily of protein-tyrosine kinase receptor ligands as studied in organ-cultured rat lungs

The Anatomical Record
S P SorokinR F Hoyt

Abstract

Macrophage precursors in pseudoglandular rat lungs rapidly differentiate into phagocytes in organ culture, although this occurs only gradually in vivo. Macrophage colony-stimulating factor is vital for the process, but the possible importance of other ligands in the platelet-derived growth factor (PDGF) subfamily is scarcely appreciated. Macrophage development was compared in 15-day fetal rat lungs cultured on solid, serum-containing media with and without added stem cell factor (SCF) (100 ng/mL) or antibodies to PDGF-AA and -BB (10-15 micrograms/mL each). In addition, organ cultures and intact lungs were immunostained for PDGF-AA and -BB to confirm their presence in the tissues. Macrophage population growth was measured by coronal area assay. SCF initially stimulated macrophage production. Thereafter, results varied depending on baseline production by control cultures: where this was vigorous, SCF-exposed explants performed similarly; where this was moderate, the SCF explants outperformed them 1.5-2.6 times over (P < 0.01-0.001). Inhibition of macrophage production by pyrrolidine dithiocarbamate (100 microM) was not significantly diminished in the presence of SCF (10 ng/mL). Immunoreactivity for PDGF-AA and -BB was prevalent i...Continue Reading

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