Aug 1, 1976

Factors influencing heat-labile Escherichia coli enterotoxin activity

Infection and Immunity
D H MundellR M Wishnow

Abstract

In this study, conditions for production, detection, and storage of heat-labile Escherichia coli enterotoxin (LT) in culture filtrates from E. coli H-10407 were defined by using the adrenal tumor cell assay system. An enriched medium containing 0.6% yeast extract, 2% Casamino Acids, and 0.25% glucose buffered at pH 8.5 produced the highest LT activity of the various test media. In E. coli strain H-10407, LT activity was markedly decreased if the initial pH of the culture media was reduced to pH 7.5 or less. In contrast to E. coli P-263, if strain H-10407 was grown in the presence of mitomycin C there was no increase in LT production. Crude-culture filtrates containing LT can be stored at 4 degrees C for several days without an appreciable loss of activity; however, for long-term storage lyophilization or freezing at -70 degrees C is recommended.

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Mentioned in this Paper

Buffers
Alkalescens-Dispar Group
Freeze Drying
Acids
Mitomycin
Assay
Escherichia coli
Entamoeba coli
YEAST extract
Enterotoxins

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