Abstract
The aim of the present study was to use the in vitro human skin sandwich system in order to quantify the influence of formulation variables on intrafollicular hydrocortisone permeation. The investigated variables were the pH and the viscosity of the topical formulation as well as the presence of chemical enhancers (carvone, menthone, oleic acid and sodium lauryl sulphate). Furthermore, skin sandwich hydration was also varied in order to determine if the method itself can be run using only partially hydrated skin tissues. It was determined that the follicular contribution to hydrocortisone flux decreased marginally with increasing alkalinity in the pH range 3-8.8. Intrafollicular penetration was markedly reduced when HPMC gels were used instead of an aqueous solution. Pretreating the skin with chemical enhancers also reduced the follicular contribution to flux, probably due to permeabilisation of the continuous stratum corneum. Furthermore, it was not possible to satisfactorily modify the skin sandwich method so that it could be deployed using less hydrated skin.
References
Sep 1, 1995·Journal of Pharmaceutical Sciences·M E JohnsonR Langer
Nov 8, 2001·The Journal of Pharmacy and Pharmacology·G M El MaghrabyB W Barry
Jul 13, 2002·Journal of Pharmaceutical Sciences·H K VaddiS Y Chan
Nov 22, 2002·Journal of Drug Targeting·Taro OgisoTetsuyuki Wada
Dec 4, 2002·Advanced Drug Delivery Reviews·B W Barry
Dec 24, 2002·The Journal of Pharmacy and Pharmacology·Ebtessam A EssaBrian W Barry
Apr 28, 2005·The Journal of Investigative Dermatology·Sujatha DokkaJames G Karras
Aug 17, 2005·International Journal of Pharmaceutics·Gul M KhanVictor M Meidan
Nov 2, 2005·International Journal of Pharmaceutics·Victor M MeidanBozena B Michniak
Feb 8, 2006·Colloids and Surfaces. B, Biointerfaces·Yonghui Yuan, Ritu Verma
Apr 7, 2006·The Journal of Pharmacy and Pharmacology·Gamal M M El MaghrabyBrian W Barry
Apr 29, 2006·The Journal of Investigative Dermatology·Sascha JungJürgen Lademann
May 11, 2006·Skin Pharmacology and Physiology·A TeichmannJ Lademann
Dec 16, 2006·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Juergen LademannWolfram Sterry
Dec 26, 2006·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Yakov FrumVictor M Meidan
Feb 3, 2007·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·L KangS Y Chan
Mar 31, 2007·Skin Pharmacology and Physiology·N OtbergJ Lademann
Sep 14, 2007·Skin Pharmacology and Physiology·U JacobiJ Lademann
Oct 20, 2007·Mycoses·M OssadnikS Koch
Citations
Mar 3, 2012·Biological & Pharmaceutical Bulletin·Tomohiro HikimaKakuji Tojo
Apr 3, 2013·Expert Opinion on Drug Delivery·Sara Caliço RaposoHelena Margarida Ribeiro
Jul 17, 2010·Expert Opinion on Drug Delivery·Victor M Meidan
Sep 28, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Ana BeloquiVéronique Préat
Jan 3, 2012·International Journal of Pharmaceutics·Omar Sarheed, Yakov Frum
Jun 10, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ana M Barbero, H Frederick Frasch
Jul 15, 2020·Pharmaceutics·Ana SimõesCarla Vitorino
Sep 1, 2016·Irish Journal of Psychological Medicine·M Osman, M D McCauley