Factors Influencing in Vitro Organogenesis of Chrysanthemum morifolium cv. 'Resomee Splendid'

Iranian Journal of Biotechnology
Rezvanolsadat KazeroonianMasoud Tohidfar

Abstract

Chrysanthemum; also commonly known as mums or chrysanths, is one of the most important ornamental crops worldwide. Introducing desirable traits into this valuable plant by the conventional breeding has so far been faced with some restrictions due to the limited gene pool and cross-incompatibility. Therefore, breeders have decided to exploit Agrobacterium-mediated transformation methods in order to satisfy the growing market demands. However, more efficient in vitro regeneration protocols are required for this approach. The objective of this research was to develop an efficient protocol for an in vitro plant regeneration by the examining the effects of various combinations and concentrations of the plant growth regulators (PGRs) and different explants types. The leaf and petiole explants of the Chrysanthemum morifolium cv. 'Resomee Splendid' were collected from the in vitro grown plantlets. Murashige and Skooge (MS) medium was supplemented with different concentrations and combinations of benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and thidiazuron (TDZ). Thereafter, the effects of these hormonal treatments were investigated on shoot initiation percentage, the average number of shoots per explants, callogenesis, and t...Continue Reading

References

Oct 1, 2013·The Plant Cell·Momoko IkeuchiAkira Iwase
Mar 3, 2015·Journal of Plant Research·Munetaka Sugiyama
May 5, 2016·Development·Momoko IkeuchiKeiko Sugimoto

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