PMID: 7528541Dec 30, 1994Paper

Factors influencing macrophage activation by muramyl peptides: inhibition of NO synthase activity by high levels of NO

Biochimica Et Biophysica Acta
C MorinG Barratt

Abstract

Treatment with muramyldipeptide (MDP) or a lipophilic derivative (MTP-Chol) included in nanocapsules renders macrophages cytostatic towards tumor cells. At the same time, nitric oxide (NO) synthase (EC 1.14.23) activity is induced, as determined by measurement of the two end products of the reaction (nitrite and L-citrulline). The objective of this study was to investigate some factors which might influence this activation and explain the decreased response observed at high nanocapsule concentrations. The glucose content of the medium did not seem to be limiting. Addition of indomethacin decreased nitrite production in the effector phase, suggesting a role for prostaglandins in the maintenance of the activated state. We also tested the hypothesis that NO itself might regulate inducible nitric oxide synthase activity. The addition of NO donors (SIN-1 and nitrosoglutathione) or superoxide dismutase to cultures of activated macrophages inhibited the NO synthase activity. Since these NO donors were non toxic towards macrophages, these observations indicate clearly that the addition of exogenous NO to that formed by the enzymatic reaction can cause inhibition of the inducible NO synthase.

References

Nov 15, 1992·The Biochemical Journal·P KlattB Mayer
Nov 30, 1992·Biochemical and Biophysical Research Communications·N E Rogers, L J Ignarro
Aug 30, 1991·Biochemical and Biophysical Research Communications·M LepoivreM Fontecave
Jan 1, 1991·International Journal of Immunopharmacology·G M BarrattJ P Tenu
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Mar 1, 1993·British Journal of Pharmacology·J AssreuyS Moncada
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D SalveminiP Needleman
Jul 15, 1993·Biochemical and Biophysical Research Communications·T InoueT Ogihara
Apr 24, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Vivien MakKerry J Savage

❮ Previous
Next ❯

Citations

Oct 22, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·S Barbault-FoucherA Bochot
Nov 7, 2000·International Journal of Pharmaceutics·N S Santos-MagalhãesM N Caetano
Jan 27, 1999·Thyroid : Official Journal of the American Thyroid Association·M E CostamagnaA M Masini-Repiso
Jan 21, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·A BreitenbachT Kissel
Dec 8, 2007·Clinical and Experimental Immunology·L Hobson, M L Everard
May 5, 1999·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·W P YuT M Chang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.