Factors influencing neuronal growth in primary cultures derived from 3-day-old chick embryos

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
D MangouraA Vernadakis

Abstract

We compared neuronal growth patterns in primary cultures prepared by dissociating 3-day-old chick embryos, either whole embryo (E3WE) or head only (E3H) and plating the dispersed cells onto Petri dishes coated with either poly-L-lysine, collagen or laminin. The culture medium was Dulbecco's Modified Eagle's Medium (DMEM), supplemented with either 5 or 10% fetal bovine calf serum (FCS). As we have previously described, in E3WE cultures on poly-L-lysine the neuronal primary growth patterns were aggregation with neuritic fasciculation, presence of growth cones with microspikes and very few flat cells. In contrast with cultures grown on poly-L-lysine, in cultures grown on collagen or laminin the distinct growth pattern was extensive networks of isolated and differentiated neurons lying on acquired monolayers of flat cells. When 5% FCS was used, as compared to 10% FCS, neuronal aggregates were fewer and smaller on poly-L-lysine; on collagen or laminin a tendency to aggregate was observed. Several differences were observed in the E3H cultures when compared to E3WE: (a) aggregates were less numerous with the prevailing pattern being a web-like, self-contained aggregate; (b) aggregates connected with other aggregates or flat cells were...Continue Reading

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Citations

Nov 1, 1990·Brain Research. Developmental Brain Research·S Kentroti, A Vernadakis
Nov 1, 1990·Brain Research. Developmental Brain Research·C Brodie, A Vernadakis
Jan 1, 1990·Brain Research. Developmental Brain Research·D MangouraA Vernadakis
Aug 19, 1991·Brain Research. Developmental Brain Research·S Kentroti, A Vernadakis
Dec 18, 1992·Brain Research. Developmental Brain Research·S KentrotiA Vernadakis
Jan 1, 1989·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A Bignami, D Dahl
Jan 1, 1991·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C Brodie, A Vernadakis
Dec 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D DahlV H Gilad
Aug 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M KozlovaA Vernadakis
Aug 1, 1994·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·H RahmanA Vernadakis
Jan 1, 1990·Annals of the New York Academy of Sciences·A VernadakisD Mangoura
Nov 1, 1989·Journal of Neuroscience Research·N SakellaridisA Vernadakis
May 26, 2007·Journal of Neuroscience Research·Helena DeckerCarla I Tasca
Jan 1, 1990·Virchows Archiv. A, Pathological Anatomy and Histopathology·I IshiwataH Ishikawa
Jul 1, 1990·Journal of Neuroscience Research·A Vernadakis, S Kentroti

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