Factors influencing proliferation and histamine content of cultured human bone marrow cells.

Immunobiology
S BrantschenB M Stadler

Abstract

Various human lymphokines such as semipurified human interleukin 3 (IL 3), recombinant human IL 3, granulocyte colony stimulating factor (G-CSF), and recombinant human interleukin 4 (IL 4) stimulated growth of human bone marrow cells, but from all these factors tested, only IL 3 by itself was able to cause an increase in histamine content. Fibroblast monolayers as well as factors in their supernatants also increased proliferation and histamine content of bone marrow cells. Concentrated supernatants (Mr greater than 10,000) also inhibited cell proliferation and induced histamine content. The same fraction concentrated on a Mr cut-off greater than 50,000 enhanced cell growth and the total histamine content per culture. Thus, fibroblast supernatants contained both growth promoting and inhibitory factors. However, using the rat basophilic leukemia (RBL) cell line as a test system for such fibroblast-derived differentiation factors, we showed that if cell proliferation was inhibited, histamine content was also enhanced. Furthermore, certain drugs known to inhibit cell division, such as sodium butyrate or hydroxyurea, were also found to cause an increase in histamine content of RBL cells. Thus, our data demonstrate that basophil/mast...Continue Reading

References

Jan 1, 1979·Experimental Cell Research·Y MoriM Okada
Jun 1, 1975·Journal of Immunological Methods·R P Siraganian
Jan 1, 1987·International Archives of Allergy and Applied Immunology·B M StadlerA L de Weck
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H SaitoT Ishizaka
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F Levi-SchafferR L Stevens
Jan 1, 1987·The Journal of Experimental Medicine·Y HamaguchiY Kitamura
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Dec 1, 1982·The Journal of Allergy and Clinical Immunology·J BienenstockR Goodacre
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M OgawaT Ishizaka
Sep 1, 1983·The Journal of Experimental Medicine·K TadokoroA L De Weck
Jan 1, 1983·Monographs in Allergy·J BienenstockF Shanahan
May 1, 1983·Scandinavian Journal of Gastroenterology·R V Heatley
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E RazinR A Good
Apr 1, 1984·Immunology Today·E E Jarrett, D M Haig

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Citations

Apr 1, 1989·Current Opinion in Immunology·K P Mathews
Jul 1, 1992·Current Problems in Cancer·G D Demetri

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