PMID: 6170887May 1, 1981Paper

Factors influencing sister-chromatid exchange rate in cultured human lymphocytes

Mutation Research
W F Morgan, P E Crossen

Abstract

Factors influencing sister-chromatid exchange (SCE) frequency in human lymphocytes were investigated. Slides treated by the hot PO4 technique showed a lower SCE rate than those treated by the fluorescence plus Giemsa (FPG) technique. Lymphocytes cultured in McCoy's 5A culture medium showed a lower SCE rate than those cultured in TC 199. Neither of the 2 batches of serum tested (Gibco batch 092 and human AB) increased the SCE rate. Cultures harvested at 48 and 72 h showed similar SCE rates. The mean SCE rate in lymphocytes from 100 subjects was 10.98 with a standard deviation of 1.71. Only 3 donors fell outside the 95% confidence level. The distribution of SCE in individual cells was judged to differ significantly from normal. Cells with increased SCE contributed to the positive skewness of the distribution. Repeat cultures from 20 subjects were studied over a 3-year period. Only 3 subjects showed significant variation in SCE in successive cultures.

References

Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G W ZackS A Latt
Jan 1, 1979·Human Genetics·R BecherD K Hossfeld
Dec 1, 1979·Mutation Research·N O BianchiM Larramendy
Feb 1, 1977·Mutation Research·W F Morgan, P E Crossen
Oct 15, 1977·Experimental Cell Research·H Kato, A A Sandberg
Jan 1, 1974·Chromosoma·J R Korenberg, E F Freedlender

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Citations

Oct 1, 1988·Human Genetics·T LukusaJ J Cassiman
Sep 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A K SinhaB B Gollapudi
Oct 1, 1982·Mutation Research·P E Crossen, W F Morgan
May 1, 1987·Mutation Research·P Morales-RamírezT Vallarino-Kelly
Sep 1, 1990·Mutation Research·P Morales-RamírezT Vallarino-Kelly
Jan 9, 1992·European Journal of Obstetrics, Gynecology, and Reproductive Biology·H HamamyB M al-Allak
Dec 1, 1992·Mutation Research·P Morales-RamírezT Vallarino-Kelly
Aug 1, 1995·Mutation Research·E CarbonellR Marcos
Aug 1, 1983·Cancer Genetics and Cytogenetics·A GhidoniN Cascinelli
Jun 1, 1988·Cancer Genetics and Cytogenetics·M I MelaragnoC M Erwenne
Jul 15, 1996·Cancer Genetics and Cytogenetics·P K DharK R Bhat
May 1, 1983·Mutation Research·L LambertiG Ardito
Sep 1, 1983·Mutation Research·A Baret, I Emerit
Oct 1, 1992·Mutation Research·Z Zhang, J Yang
Jun 23, 2016·Arhiv za higijenu rada i toksikologiju·Rafael Valencia-QuintanaMaría Elena Calderón-Segura
Jul 3, 1999·Journal of Occupational and Environmental Medicine·B Gollapudi, C Burns

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