Factors Influencing the Activity of Nanozymes in the Cleavage of an RNA Model Substrate

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Joanna CzescikPaolo Scrimin

Abstract

A series of 2-nm gold nanoparticles passivated with different thiols all featuring at least one triazacyclonanone-Zn(II) complex and different flanking units (a second Zn(II) complex, a triethyleneoxymethyl derivative or a guanidinium of arginine of a peptide) were prepared and studied for their efficiency in the cleavage of the RNA-model substrate 2-hydroxypropyl-p-nitrophenyl phosphate. The source of catalysis for each of them was elucidated from the kinetic analysis (Michaelis-Menten profiles, pH dependence and kinetic isotope effect). The data indicated that two different mechanisms were operative: One involving two Zn(II) complexes and the other one involving a single Zn(II) complex and a flanking guanidinium cation. The mechanism based on a dinuclear catalytic site appeared more efficient than the one based on the cooperativity between a metal complex and a guanidinium.

References

Aug 12, 2004·Journal of the American Chemical Society·Anna M PiatekEric V Anslyn
Nov 19, 2004·Angewandte Chemie·Flavio ManeaPaolo Scrimin
Apr 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andrew D Hughes, Eric V Anslyn
May 15, 2007·Journal of the American Chemical Society·Marco MartinPaolo Scrimin
Feb 12, 2008·Current Opinion in Chemical Biology·Cynthia M Dupureur
Mar 18, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Flavio ManeaPaolo Scrimin
Nov 4, 2008·Journal of the American Chemical Society·Renato BonomiPaolo Scrimin
Feb 24, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Zhiming ZhangJunqiu Liu
Feb 19, 2010·Angewandte Chemie·Charles E Hoyle, Christopher N Bowman
May 21, 2010·Organic & Biomolecular Chemistry·Renato BonomiFabrizio Mancin
Oct 1, 2011·Chemical Communications : Chem Comm·Gaetano GuarinoFabrizio Mancin
Mar 31, 2012·Journal of the American Chemical Society·Gaetano GuarinoFabrizio Mancin
May 1, 2012·Chemical Communications : Chem Comm·Fabrizio MancinPaolo Tecilla
Sep 18, 2013·Journal of the American Chemical Society·Danielle C LohmanRichard Wolfenden
Jan 11, 2014·Journal of the American Chemical Society·Marta Diez-CastellnouPaolo Scrimin
Jun 13, 2014·Angewandte Chemie·Emmanuel Y TirelNicholas H Williams
Jan 16, 2015·Accounts of Chemical Research·Giulia PalermoMarco De Vivo
Aug 17, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fabrizio MancinPaolo Scrimin
Aug 5, 2017·Chem·Laura RiccardiMarco De Vivo
Aug 5, 2017·Accounts of Chemical Research·Quy OngFrancesco Stellacci

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Citations

Apr 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Felix Zellmann, Michael W Göbel
Sep 29, 2020·Angewandte Chemie·Joanna CzescikPaolo Scrimin
Mar 28, 2020·Journal of the American Chemical Society·Joanna CzescikFabrizio Mancin
Mar 28, 2021·Journal of Hazardous Materials·Yanchao LyuPaolo Scrimin
Apr 20, 2021·Chemistry : a European Journal·Ying-Juan CaoJiang-Shan Shen
Mar 30, 2021·Chemistry : a European Journal·Joanna CzescikPaolo Scrimin

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Methods Mentioned

BETA
transmission electron microscopy
kinetic titration

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