PMID: 2121940Jun 1, 1990Paper

Factors influencing the distribution pattern of porphyrins in cell membranes

Journal of Photochemistry and Photobiology. B, Biology
F RicchelliC Salet

Abstract

The mechanism of the sensitizer-membrane interactions has been studied by following the distribution properties of selected porphyrins, including haematoporphyrin (HP) and protoporphyrin (PP), into unilamellar liposomes of dipalmitoyl phosphatidylcholine (DPPC). The endomembrane distribution of HP and PP has been checked as a function of the membrane fluidity and composition by fluorescence polarization and quenching techniques. At porphyrin concentrations below 0.5 microM, HP and PP exclusively localize in the inner phospholipid monolayer; at higher concentrations, the outer monolayer also becomes populated. The porphyrin binding sites in liposomes, however, are different for HP and PP: HP preferentially distributes into water-accessible lipid regions, while PP localizes in the most hydrophobic loci of the lipid matrix. A porphyrin redistribution occurs when the fluidity properties of the liposomes are changed by addition of cholesterol or cardiolipin. In DPPC-cholesterol vesicles, all HP molecules dissolve in DPPC-rich regions while all PP molecules partition in cholesterol-rich environments. In DPPC-cardiolipin vesicles both porphyrins preferentially localize in regions accessible to the external medium. The effect of the na...Continue Reading

Citations

Aug 2, 1996·Cancer Letters·A K HaylettJ V Moore
Feb 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·V M MirskyT V Szitó
Jan 1, 1994·Journal of Photochemistry and Photobiology. B, Biology·S GeorgiouA Tosca
Nov 1, 1994·Journal of Photochemistry and Photobiology. B, Biology·N CempelD Guillochon
Aug 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·F Ricchelli
Nov 22, 2007·Photochemistry and Photobiology·Yongqing WeiQifeng Yang
Mar 4, 1998·Lasers in Surgery and Medicine·F JiangM Chopp
Apr 4, 2017·Journal of Inorganic Biochemistry·Rainer LippertIvana Ivanović-Burmazović
May 1, 1992·Photochemistry and Photobiology·K W WoodburnD R Phillips
Jan 6, 2000·Archives of Biochemistry and Biophysics·R GalántaiJ Fidy
May 31, 1991·Biochimica Et Biophysica Acta·F RicchelliM Tronchin

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