PMID: 39418Jan 1, 1978

Factors influencing the uptake of DNA by Neurospora crassa

Acta Biochimica Et Biophysica; Academiae Scientiarum Hungaricae
J AradiG Szabó

Abstract

Under optimal conditions intact Neurospora crassa cells incorporated nearly the same amount of 3H-labelled DNA as that of the endogenous DNA content of cells. After 18 h of incorporation more than 80 per cent of the radioactivity was retained in the cells. A maximum uptake of exogenous DNA occurred at 28 degrees C, pH 6.35, in the presence of 100 mM calcium when the concentration of donor DNA was 150 micrograms/ml. Denatured DNA was incorporated at a higher rate than native DNA. The present findings suggest that the uptake of DNA by Neurospora crassa is a biologically active process.

Related Concepts

DNA, Double-Stranded
Hydrogen-Ion Concentration
Neurospora
Neurospora crassa
RNA Denaturation

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