PMID: 6402761Mar 11, 1983Paper

Factors involved in the initiation of phage phi 29 DNA replication in vitro: requirement of the gene 2 product for the formation of the protein p3-dAMP complex

Nucleic Acids Research
L BlancoM Salas

Abstract

To study the requirements for the in vitro formation of the protein p3-dAMP complex, the first step in phi29 DNA replication, extracts from B. subtilis infected with phi29 mutants in genes 2, 3, 5, 6 and 17, involved in DNA synthesis, have been used. The formation of the initiation complex is completely dependent on the presence of a functional gene 2 product, in addition to protein p3 and phi29 DNA-protein p3 as template. ATP is also required, although it can be replaced by other nucleotides. The products of genes 5, 6 and 17 do not seem to be needed in the formation of the initiation complex. Inhibitors of the host DNA polymerase III, DNA gyrase or RNA polymerase had no effect on the formation of the protein p3-dAMP complex, suggesting that these proteins are not involved in the initiation of phi29 DNA replication. ddATP or aphidicolin, inhibitors of DNA chain elongation, had also no effect on the formation of the initiation complex.

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Citations

Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J A GarcíaM Salas
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L Blanco, M Salas
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K WatabeJ Ito
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·I PrietoM Salas
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L Blanco, M Salas
Dec 10, 1983·Nucleic Acids Research·K Watabe, J Ito
Jan 1, 1984·CRC Critical Reviews in Biochemistry·K J Marians
Sep 28, 1984·Biochemical and Biophysical Research Communications·K WatabeJ Ito
Apr 12, 1996·The Journal of Biological Chemistry·L Blanco, M Salas

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