PMID: 2494280Mar 1, 1989Paper

Factors mediating lipopolysaccharide-induced ganglioside expression in murine peritoneal macrophages

Journal of Leukocyte Biology
C S BerensonJ L Ryan

Abstract

The ganglioside composition of endotoxin-responsive C3H/HeN murine peritoneal macrophages is known to undergo dramatic changes in vivo in response to intraperitoneal lipopolysaccharides (LPS), unlike endotoxin-hyporesponsive C3H/HeJ macrophages. To better investigate the mechanism behind LPS-induced macrophage ganglioside changes, resident C3H/HeN peritoneal macrophages were treated in vitro with 0.1-1.0 micrograms/ml LPS for 6-96 hr, but showed no differences in membrane ganglioside patterns. Coincubation of macrophages with lymphocytes and treating with LPS again elicited no ganglioside changes. In contrast, interferon gamma (IFN-gamma)-primed macrophages showed a dramatic shift in intensity of one ganglioside when treated with LPS in vitro; an additional macrophage ganglioside appeared when IFN-gamma-primed, LPS-treated macrophages were coincubated with lymphocytes. Ganglioside expression induced in vitro still did not approach the complex changes seen in vivo. However, transplanting C3H/HeN macrophages intraperitoneally into C3H/HeJ mice, followed by administration of intraperitoneal LPS, did reveal striking changes in ganglioside expression that resembled the pattern seen in vivo. Thus, LPS alone does not provide the neces...Continue Reading

Citations

Mar 11, 1998·Molecular and Chemical Neuropathology·T N SeyfriedH C Yohe
Feb 1, 1990·Immunological Investigations·A D YurochkoK D Elgert
Jan 19, 2018·Journal of Leukocyte Biology·Elena ChiricozziMassimo Aureli

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