PMID: 6161677Jan 1, 1981Paper

Factors modulating the response of a porcine renal tubular cell line to calcitonin and antidiuretic hormone

Calcified Tissue International
J F HéronS M Krane

Abstract

A cell line (LLC-PK1) isolated from porcine kidney increases 3'5'-cyclic adenosine monophosphate (cAMP) content when incubated with salmon calcitonin (SCT) or antidiuretic hormone (ADH). We have examined several factors which modulate the hormone-induced changes in cAMP levels in these cells. Preincubation with increasing concentrations of SCT results in a dose-dependent decrease in cAMP levels in cells retested with this hormone. Addition of 3-isobutyl-l-methylxanthine (IBMX) to cells preincubated with SCT results in 15--30-fold increases in cAMP levels compared to cells preincubated without this hormone. These observations suggest that the decrease in SCT-induced cAMP response in cells pretreated with this hormone is related at least in part to stimulation of phosphodiesterase activity. Preincubation with ADH does not affect subsequent cAMP response to either ADH or SCT, suggesting that these hormones interact with different cell surface receptors. Cell cycle and plating density also affect cAMP levels. cAMP content per cell increases with increasing cell density, which is associated with an increase in the SCT-induced cAMP response. These studies illustrate that factors other than receptor occupancy modulate cAMP responses o...Continue Reading

References

Jan 13, 1978·Science·P Greengard
Mar 1, 1978·The Journal of Clinical Endocrinology and Metabolism·S R GoldringS M Krane
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D S NewcombeJ V Fahey
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J MoranJ A Fischer
Jul 28, 1978·Biochemical and Biophysical Research Communications·S R GoldringS M Krane
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J PénitS Jard
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·C MukherjeeR J Lefkowitz
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J P Schwartz, J V Passonneau
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·W Seifert, P S Rudland
Jan 1, 1964·Cold Spring Harbor Symposia on Quantitative Biology·T T PUCK
May 13, 1977·Science·G B Kolata

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Citations

Oct 1, 1981·The Journal of Cell Biology·J M DayerS M Krane
Jan 1, 1985·Annals of the New York Academy of Sciences·H MurerM Mohrmann
Jun 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K S UpchurchS M Krane
Jan 1, 1985·The Journal of Membrane Biology·J F García-DíazA Essig
Nov 1, 1985·Circulation Research·B ZimmerhacklR L Jamison

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