PMID: 7011813Feb 1, 1981Paper

Factors modulating transcription and translation in vitro of ribosomal protein S20 and isoleucyl-tRNA synthetase from Escherichia coli

European Journal of Biochemistry
R WirthA Böck

Abstract

The DNA-dependent protein-synthesizing system developed by Zubay [Zubay, G. (1973) Annu. Rev. Genet. 7, 267--287] was optimized for the transcription and translation of genes from the 0.5-min region of the Escherichia coli chromosome carried by transducing lambda phages. The E. coli gene products synthesized were isoleucyl tRNA synthetase, ribosomal protein S20, dihydrodipicolinic acid reductase and (possibly) the two subunits carbamoyl-phosphate synthetase. Formation of ribosomal protein S20 is specifically stimulated by the addition of 16-S rRNA and not by 5-S or 23-S rRNA. 16-S rRNA increases the rate of S20 synthesis, the final yield of product depends on the duration of persistence of the RNA added. Addition of 16-S rRNA to the separate transcription and translation systems showed that it is the translation of the S20 mRNA which is enhanced. Furthermore, S20 synthesis is stimulated more than fourfold when concomitant synthesis of rRNA occurs from a plasmid carrying an rrn transcriptional unit. The results described are explained in terms of a model which suggests that ribosomal protein S20 feedback inhibits its synthesis at the translational level and that removal of S20 into ribosomal assembly (i.e. binding to 16-S rRNA) ...Continue Reading

References

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Citations

Jan 1, 1981·Molecular & General Genetics : MGG·A Böck
Feb 18, 2016·Molecular Biology and Evolution·Anna KnöppelDan I Andersson
Sep 18, 2009·PloS One·Mark WelchClaes Gustafsson
Jan 1, 1983·Zeitschrift für allgemeine Mikrobiologie·K Riedel
Apr 1, 1983·Journal of Bacteriology·G D Parsons, G A Mackie

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