Faecal calprotectin determination: impact of preanalytical sample treatment and stool consistency on within- and between-method variability

Biochemia Medica
Gordana JuricicLorena Honovic

Abstract

We assessed the differences in faecal calprotectin (FC) concentrations measured by two assays depending on the stool consistency and extraction method. Stool samples were extracted using the EliA Stool Extraction Kit, Calex® Cap extraction device and respective weighing methods, while FC concentrations were measured using the EliATM Calprotectin and Bühlmann fCAL® Turbo method and checked for within- and between-method variability with regard to extraction method and stool consistency category. Extraction yield was evaluated for impact of different sample incubation time (10 min and 1 h) in extraction buffer for both methods and for impact of different initial sample dilutions (1:50, 1:100, 1:500) for fCAL® Turbo method. Results determined from Calex® Cap extracts were higher compared to weighing method extracts (mean bias 33.3%; P < 0.001), while no significant difference was found between results obtained with EliA Stool Extraction Kit and weighing method (mean bias 0.1%; P = 0.484), in both cases irrespective of stool consistency. Bühlmann fCAL® Turbo results were higher than EliATM Calprotectin results (mean bias 32.3%, P = 0.025 weighing method; and mean bias 53.9%, P < 0.001 extraction devices), the difference is dependen...Continue Reading

References

Sep 1, 1992·Scandinavian Journal of Gastroenterology·A G RøsethH Schjønsby
Feb 25, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·H TønB Johne
Jun 12, 2014·United European Gastroenterology Journal·Delphine LabaerePeter Bossuyt
Apr 10, 2015·Scandinavian Journal of Clinical and Laboratory Investigation·Vendel KristensenBjØrn Moum
Jan 27, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Vendel KristensenBjørn Moum
Jan 12, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Maxime M W De SloovereHilde J M Vanpoucke
Feb 22, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Matthijs OyaertLieve Van Hoovels
May 17, 2017·Digestion·Fabrizia D'AngeloJean Louis Frossard
Aug 19, 2017·Medical Education Online·Abdulla BedirHaleema Aya
Aug 30, 2017·Clinica Chimica Acta; International Journal of Clinical Chemistry·Matthijs OyaertLieve Van Hoovels
Nov 10, 2017·Medical Education Online·Shou Ling LeongMartin Pusic
May 8, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Andrea PadoanDaniela Basso
Jan 1, 2018·The European Physical Journal. C, Particles and Fields·L A Harland-LangR S Thorne

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Citations

May 12, 2020·Journal of Crohn's & Colitis·Ferdinando D'AmicoLaurent Peyrin-Biroulet
May 21, 2020·Annals of Clinical Biochemistry·Sukhbir KaurRousseau Gama
Feb 15, 2021·The Journal of Applied Laboratory Medicine·Sara P WynessLisa M Johnson
May 8, 2021·United European Gastroenterology Journal·Ferdinando D'AmicoLaurent Peyrin-Biroulet
Aug 15, 2021·American Journal of Clinical Pathology·Leo LinLisa M Johnson

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Methods Mentioned

BETA
biopsy

Software Mentioned

MedCalc

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