Failure of multitube sperm swim-up for sex preselection

Fertility and Sterility
C J De JongeC D Matthews

Abstract

To use double-label fluorescence in situ hybridization to evaluate a modified swim-up procedure that is purported to be effective for preconceptual sex selection. Controlled, blinded study. University hospital laboratories. Donor males reporting for routine semen analysis. Percentages of X- and Y-bearing spermatozoa in neat semen and in two swim-up fractions, determined using double-label fluorescence in situ hybridization. No clinically significant change from a 1:1 ratio was found in the distribution of X- or Y-bearing spermatozoa after double-label fluorescence in situ hybridization following a modified swim-up procedure and irrespective of the time (15, 30, 45, and 60 minutes) allowed for swim-up. Using fluorescence in situ hybridization, a modified swim-up procedure was evaluated for its purported ability to skew the relative percentages of X- and Y-bearing spermatozoa. No clinically significant change in the ratio of X- to Y-bearing spermatozoa was detected independent of time. Therefore, clinical application of this procedure should be strongly discouraged.

References

Dec 1, 1989·Fertility and Sterility·P W ZarutskieM R Soules
Dec 14, 1973·Nature·R J EricssonM Nishino
Mar 1, 1993·Molecular Reproduction and Development·T L HanC D Matthews
Sep 1, 1996·Fertility and Sterility·B E Reubinoff, J G Schenker
Dec 1, 1996·Molecular Human Reproduction·S P Flaherty, C D Matthews

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Citations

Mar 4, 1999·The Journal of Obstetrics and Gynaecology Research·P Li, H Hoshiai
Jun 25, 2014·Reproductive Biology and Endocrinology : RB&E·Juan J TarínAntonio Cano

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