PMID: 9550324Apr 29, 1998Paper

Failure of TGF beta1 and IL-12 to regulate human FasL and mTNF alloreactive cytotoxic T-cell pathways

Tissue Antigens
C Asselin-PaturelS Chouaib

Abstract

The effect of TGFbeta1 and IL-12 on calcium-independent cytotoxic pathways was investigated. We have previously demonstrated that the regulatory effect of TGFbeta1 and IL-12 on human alloreative CTL activity was associated with regulation of perforin and granzyme B gene expression. To determine the effect of both cytokines on the alternative cytotoxic pathway involving FasL and mTNF, we first investigated the expression of both molecules on human primary alloactivated T cells. Our results show that human allostimulated T lymphocytes express FasL. Cell lysis experiments demonstrate that the FasL cytotoxic pathway is involved in the killing of specific target cells mediated by human alloreactive CTL. In addition, allogeneic stimulation induced significant mTNF expression on both CD4+ and CD8+ responder T cells. Using TNF-sensitive target cells, we also demonstrate that the mTNF-mediated cytotoxic pathway is involved in the cytotoxic activity of human primary allostimulated T lymphocytes. Neither TGFbeta1 nor IL-12 had an effect on FasL or mTNF expression. Furthermore, addition of TGFbeta1 or IL-12 at the initiation of the MLR had no significant effect on Fas- and mTNF-mediated cytotoxicity. Taken together, our results provide a n...Continue Reading

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Citations

Jul 13, 2000·Microscopy Research and Technique·M J Smyth, R W Johnstone
Jan 29, 2011·Cancer Metastasis Reviews·Catherine Sautès-FridmanMarie-Caroline Dieu-Nosjean
Oct 3, 2002·International Immunology·Séverine BlessonSalem Chouaib
Mar 26, 2003·The American Journal of Pathology·Dominique ChopinFrancesca Velotti

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