Failure of the most-probable-number technique to detect coliforms in drinking water and raw water supplies.

Applied and Environmental Microbiology
T M EvansM W LeChevallier

Abstract

A procedure was developed to detect false-negative reactions (interference) in the standard most-probable-number (S-MPN) technique for coliform enumeration of untreated surface water and potable water supplies. This modified MPN (M-MPN) procedure allowed a quantitative assessment of the interference with coliform detection in untreated surface water and potable water supplies. Coliform interference was found to occur in the presumptive, confirmed, and completed tests of the S-MPN technique. When coliforms were present, interference with their detection occurred in over 80% of the samples. The inferior nature of the S-MPN was revealed by the 100% increase in the incidence of completed coliform-positive drinking water samples obtained with the M-MPN technique. The M-MPN procedure was also superior to the standard membrane filter technique. Eight different species of coliforms were recovered from false-negative tests, including Citrobacter, Enterobacter, Klebsiella, and Escherichia coli (in decreasing order of occurrence). The use of standard MPN techniques for monitoring potable water supplies may lead to a false security that the drinking water supply is potable, i.e., free from indicator bacteria.

References

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Citations

Jan 5, 2002·Journal of Microbiological Methods·Annie RompréPatrick Laurent
Oct 1, 1984·Applied and Environmental Microbiology·R G QuallsJ D Johnson
Feb 1, 1983·Applied and Environmental Microbiology·M W LeChevallierG A McFeters
Oct 1, 1982·Applied and Environmental Microbiology·J H Standridge, J J Delfino
Jul 1, 1981·Applied and Environmental Microbiology·M W LeChevallierR J Seidler
Jun 1, 1982·Applied and Environmental Microbiology·P C Feng, P A Hartman
Apr 1, 1985·Applied and Environmental Microbiology·J P Petzel, P A Hartman
Feb 1, 1990·Applied and Environmental Microbiology·S C EdbergN J Kriz

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