Failure to detect papovavirus-associated T antigens in human brain tumor cells by anticomplement immunofluorescence

Journal of Neurosurgery
H KosakaY Hinuma

Abstract

To probe the possible presence of papovavirus-related T antigen(s) in human brain tumors, the imprinted or cultured cells at various passage levels were examined by anticomplement immunofluorescence using antisera to T antigen of each BK virus, JC virus, and simian virus 40. No T antigen was demonstrated in any tests with cells derived from 69 patients with various brain tumors. Twenty-two tumor cell strains cultured in the presence of a tumor promoter, phorbol ester, also failed to show the T antigen.

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay

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