Failure to detect superoxide in human neutrophils stimulated with latex particles

Pediatric Research
J T Curnutte, A I Tauber

Abstract

Human neutrophils stimulated with either latex particles or opsonized zymosan exhibited equivalent rates of net oxygen consumption as well as hydrogen peroxide release. The quantity of superoxide (O2-) detected in latex-stimulated neutrophils was less than 2% of that seen with opsonized zymosan stimulation, and only several-fold greater than that of resting cells. The failure to detect O2- in the latex-stimulated neutrophils was due neither to latex acting as a O2- scavenger nor to its interference with the O2- -forming system of the neutrophil. An intracellular site of O2- generation could not be demonstrated. NADPH oxidase activity in cells exposed to latex particles was only 10% of that seen in cells comparably activated with opsonized zymosan. Latex particles have the unusual property of stimulating the respiratory burst of the human neutrophil without the extracellular release of O2-. The potential physiologic importance of this finding is discussed.

Citations

Nov 1, 1989·Biulleten' eksperimental'noĭ biologii i meditsiny·A N OsipovO A Azizova
Sep 1, 1988·Kidney International·N TopleyD Lloyd
Mar 30, 1990·Biochemical and Biophysical Research Communications·P Follin, C Dahlgren
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y CadroyH Plaisancié

Related Concepts

Oxydol
Latex
Latex Spheres
Neutrophil Band Cells
Dioxygen
Oxygen Consumption
Superoxides
Zymosan

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