PMID: 9546805Apr 18, 1998Paper

Failure to quantify viral load with two of the three commercial methods in a pregnant woman harboring an HIV type 1 subtype G strain

AIDS Research and Human Retroviruses
Z DebyserA M Vandamme

Abstract

The level of HIV-1 RNA in plasma has become one of the most important markers in the follow-up of HIV-infected patients. Three techniques are commercially available: both the Amplicor HIV Monitor and the NASBA HIV-1 RNA QT are target amplification methods, whereas the Quantiplex HIV RNA assay is a branched DNA signal amplification technique. Detection in both target amplification techniques is based on a single primer pair and a single probe in the gag region, whereas multiple probes capture the pol region of the viral RNA in the branched DNA assay. We investigated the discrepant observation of an undetectable viral load in an immunodeficient pregnant HIV-1-infected patient of African origin with no prior antiretroviral treatment. Although clinical progression was present in this patient with tuberculosis and a low CD4 cell count, viral load determinations with both the Amplicor Monitor and NASBA assays revealed no detectable RNA levels. The presence of HIV-1 RNA in the plasma of the patient was demonstrated by an in-house RNA-PCR. Subsequent HIV-1 RNA quantification with the branched DNA method revealed a high viremia (460,000 copies/ml). DNA sequence analysis of the gag gene identified a subtype G HIV-1 strain (HIV-1BL). To o...Continue Reading

References

Jun 1, 1990·AIDS Research and Human Retroviruses·U SauermannG Hunsmann
Dec 20, 1984·Nature·M AlizonS Wain-Hobson
Sep 30, 1995·Lancet·I Loussert-AjakaS Saragosti
Apr 15, 1995·Annals of Internal Medicine·J W MellorsP Gupta
May 1, 1994·AIDS Research and Human Retroviruses·J LouwagieD S Burke
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·G FangB Weiser
May 18, 1996·Lancet·K FransenG van der Groen
Jun 1, 1996·Nature Medicine·M S SaagP A Volberding
Oct 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·A M VandammeJ Desmyter
Feb 1, 1997·Nucleic Acids Research·C ChristophersonS Kwok
Jun 15, 1997·Annals of Internal Medicine·J W MellorsC R Rinaldo

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Citations

Jun 17, 2000·Journal of Virological Methods·I D TattJ P Clewley
Sep 21, 2000·Journal of Virological Methods·P SwansonJ Hackett
Sep 17, 1999·Journal of Virological Methods·C PasquierJ Izopet
Feb 26, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Philip CunninghamDavid Cooper
Dec 10, 1999·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·C B ChewD E Dwyer
Oct 6, 2000·AIDS Research and Human Retroviruses·A HolguínV Soriano
Apr 30, 1999·AIDS Research and Human Retroviruses·J N NkengasongS Z Wiktor
Apr 30, 1999·AIDS Research and Human Retroviruses·R B OelrichsN J Deacon
Mar 10, 2004·AIDS Patient Care and STDs·James B Peter, J Sanders Sevall
Nov 21, 1998·AIDS Research and Human Retroviruses·R B OelrichsN J Deacon
Sep 23, 2006·AIDS Research and Human Retroviruses·Gary E CorriganRigmor Thorstensson
Aug 13, 2002·AIDS·María Teresa CuevasMichael M Thomson
Oct 2, 2002·Journal of Clinical Microbiology·Mao-Yuan ChenChun-Nan Lee
Aug 6, 2005·Journal of Clinical Microbiology·Florence DamondFrancoise Brun-Vezinet
Mar 21, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Anupa KamatMahendra Kumar
Sep 18, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Vivian KouríKristel Van Laethem
Jul 20, 2011·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·LeeAnne M LuftDeirdre L Church
Dec 29, 2013·International Journal of Microbiology·Deborah SteenselsHans De Beenhouwer
Dec 11, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Alessandra AmendolaMaria R Capobianchi
Jun 8, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·J BöniJ Schüpbach
Apr 24, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·J V ParryJ P Clewley
Jun 7, 2003·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Jean-Christophe PlantierFrançois Simon
Jul 6, 2000·Journal of Clinical Microbiology·C N LeeM Y Chen

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