FAIM Opposes Aggregation of Mutant SOD1 That Typifies Some Forms of Familial Amyotrophic Lateral Sclerosis

Frontiers in Neuroscience
Hiroaki KakuThomas L Rothstein

Abstract

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative illness that is unremittingly fatal and for which no effective treatment exists. All forms of ALS are characterized by protein aggregation. In familial forms of ALS, specific and heritable aggregation-prone proteins have been identified, such as mutant superoxide dismutase (SOD1). It has been suggested that a factor capable of preventing mutant SOD1 protein aggregation and/or disassembling mutant SOD1 protein aggregates would ameliorate SOD1-associated forms of familial ALS. Here we identify Fas Apoptosis Inhibitory Molecule (FAIM), a highly evolutionarily conserved 20 kDa protein, as an agent with this activity. We show FAIM counteracts intracellular accumulation of mutant SOD1 protein aggregates, which is increased in the absence of FAIM, as determined by pulse-shape analysis and filter trap assays. In a cell-free system, FAIM inhibits aggregation of mutant SOD1, and further disassembles and solubilizes established mutant SOD1 protein aggregates, as determined by thioflavin T (ThT), filter trap, and sedimentation assays. In sum, we report here a previously unknown activity of FAIM that opposes ALS disease-related protein aggregation and promotes proteostas...Continue Reading

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Citations

Jan 12, 2021·Frontiers in Cell and Developmental Biology·Elena CocciaM José Pérez-García
Feb 23, 2021·Frontiers in Cellular Neuroscience·Marina WarepamLaishram Rajendrakumar Singh

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Methods Mentioned

BETA
Assay
Protein Assay
Fluorescence
PCR
flow cytometry
transfection
filter trap assay
NMR
X-ray

Software Mentioned

GraphPad Prism

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