FAM134B promotes esophageal squamous cell carcinoma in vitro and its correlations with clinicopathologic features

Human Pathology
Farhadul IslamAlfred King-Yin Lam

Abstract

Family with sequence similarity 134, member B (FAM134B) is an autophagy regulator of endoplasmic reticulum first discovered to be involved in the pathogenesis of esophageal squamous cell carcinoma (ESCC). The present study examined the functional behavior of FAM134B in cancer cells and the association of FAM134B expression with clinicopathologic factors in patients with ESCC. Expression at both the mRNA and protein levels was investigated using real-time polymerase chain reaction and immunohistochemistry. The results were correlated with the clinical and pathological features of the patients. In addition, in vitro functional assays were used to investigate the roles of FAM134B in ESCC cells in response to gene silencing with shRNA lentiviral particles. Overexpression of FAM134B mRNA and protein was present in 31.2% (n = 29/93) and 36.6% (n = 41/112), respectively, in tumors, whereas downregulation occurred in 39.8% (n = 37/93) and 63.4% (n = 71/112), respectively. Expression of FAM134B protein in ESCC correlated with histologic grade (P = .002) and pathologic stage (P = .012). In vitro suppression of FAM134B in ESCC induced significant reductions of cell proliferation and colony formation (P < .05). In addition, suppression of ...Continue Reading

References

Mar 29, 2000·Critical Reviews in Oncology/hematology·A K Lam
May 21, 2013·World Journal of Gastroenterology : WJG·Dessy ChanJohnny Cheuk-On Tang
Jun 15, 2014·Experimental and Molecular Pathology·Kais KasemAlfred K-Y Lam
Jun 29, 2014·Experimental Cell Research·Kais KasemAlfred K-Y Lam
Jun 5, 2015·Nature·Aliaksandr KhaminetsIvan Dikic
Apr 28, 2016·Molecular Carcinogenesis·Farhadul IslamAlfred King-Yin Lam
Jul 5, 2016·Scientific Reports·Md Hakimul HaqueAlfred King-Yin Lam
Oct 25, 2016·Experimental Cell Research·Vinod GopalanAlfred King-Yin Lam
Dec 12, 2017·Journal of Cellular Physiology·Farhadul IslamAlfred King-Yin Lam
Jan 18, 2018·Experimental and Molecular Pathology·Farhadul IslamAlfred K-Y Lam
Mar 21, 2018·European Journal of Cell Biology·Katherine Ting-Wei LeeAlfred King-Yin Lam
Mar 31, 2018·Methods in Molecular Biology·Nassim Saremi, Alfred K Lam
Mar 31, 2018·Methods in Molecular Biology·Farhadul IslamAlfred K Lam
Jun 10, 2018·International Journal of Biological Macromolecules·Farhadul IslamSyed Rashel Kabir

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Citations

Oct 13, 2020·Frontiers in Oncology·Farhadul IslamSuja Pillai
Nov 18, 2020·Cell Death & Disease·Jie MoBixiang Zhang
Oct 15, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Umur KelesMehmet Ozturk
Mar 6, 2021·Frontiers in Oncology·Plabon Kumar DasAlfred K Lam
Jun 3, 2021·International Journal of Molecular Sciences·Kamila BuzunAnna Bielawska
Jul 30, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Priti TagdeMohamed M Abdel-Daim

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