Family with sequence similarity member 20C is the primary but not the only kinase for the small-integrin-binding ligand N-linked glycoproteins in bone

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Xiudong YangXiaofang Wang

Abstract

Recent studies have identified family with sequence similarity member 20C (FAM20C) as a kinase that phosphorylates the Ser in Ser-X-Glu/phospho-Ser (pSer) motifs in the small-integrin-binding ligand N-linked glycoproteins (SIBLINGs). There is no in vivo evidence that validates this finding, and it is unclear whether FAM20C is the only kinase for SIBLINGs. We extracted bone noncollagenous proteins (NCPs) from Fam20C-knockout (KO) mice and analyzed the phosphorylation levels. The total NCPs were separated into osteopontin-, bone sialoprotein-, and dentin matrix protein-1-enriched fractions by anion-exchange chromatography and analyzed by SDS-PAGE, native PAGE, and Western immunoblot analysis. The NCP phosphorylation level in the KO mice was lower than that in the wild-type (WT). On the native gel, the SIBLINGs from KO mice showed a lower migration rate (Mr) than those from the WT. Calf intestine phosphatase treatment shifted SIBLINGs from the WT mice to the level adjacent to the KO, but failed to shift the latter, suggesting a phosphorylation loss of SIBLINGs in the KO mice. Mass spectrometry identified less pSers in the SIBLINGs from the KO mice [including the region of the acidic Ser- and aspartate-rich motif (ASARM) peptides]....Continue Reading

References

Feb 15, 1987·Analytical Biochemistry·P P Van Veldhoven, G P Mannaerts
Apr 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C Sfeir, A Veis
Oct 1, 1995·Protein Science : a Publication of the Protein Society·E S SørensenT E Petersen
Jul 12, 1996·The Journal of Biological Chemistry·E SalihM J Glimcher
Oct 7, 1997·Bone·T SaitoM A Crenshaw
Jan 1, 1997·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A VeisC B Wu
Apr 15, 2000·The Journal of Biological Chemistry·S JonoC M Giachelli
May 12, 2001·European Journal of Oral Sciences·C QinW T Butler
Jun 6, 2003·European Journal of Oral Sciences·Chunlin QinWilliam T Butler
Jun 10, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C QinW T Butler
Jul 12, 2005·Calcified Tissue International·A GerickeA L Boskey
Mar 21, 2008·European Journal of Oral Sciences·Bingzhen HuangChunlin Qin
Jun 13, 2009·Biochemistry·Paul CurtinErdjan Salih
Sep 25, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·William N AddisonMarc D McKee
Jul 21, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Xiaofang WangChunlin Qin
Feb 22, 2012·Critical Reviews in Eukaryotic Gene Expression·Peter S N Rowe
Aug 14, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Baozhi YuanMarc K Drezner
Sep 1, 2012·The Journal of Biological Chemistry·Xiaofang WangChunlin Qin
Sep 20, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nilana M T BarrosMarc D McKee
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Vincent S TagliabracciJack E Dixon
Aug 30, 2014·Cell·Mattia R BordoliMalcolm Whitman
Mar 27, 2015·Molecular Endocrinology·Bruno Ramos-Molina, Iris Lindberg

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Citations

Nov 12, 2016·Molecular & Cellular Proteomics : MCP·Eva Klement, Katalin F Medzihradszky
Mar 30, 2018·International Journal of Oral Science·Wen-Juan YanXiao-Fang Wang
Jul 12, 2017·Frontiers in Physiology·Claire E L SmithAlan J Mighell
Feb 28, 2019·Orphanet Journal of Rare Diseases·Signe Sparre Beck-NielsenOuti Mäkitie
Aug 18, 2020·JBMR Plus·Brian ChristensenEsben S Sørensen
Apr 27, 2017·Biophysical Journal·Samuel LentonSusana C Marujo Teixeira
Apr 23, 2021·Calcified Tissue International·Nattanan GovitvattanaYoshiyuki Mochida
Apr 28, 2021·European Journal of Oral Sciences·Hua ZhangChunlin Qin
Aug 8, 2021·International Journal of Molecular Sciences·Icela Palma-LaraCarmen Palacios-Reyes

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