Fangchinoline inhibits migration and causes apoptosis of human breast cancer MDA-MB-231 cells

Oncology Letters
Binggao WangYanhui Zhang

Abstract

In order to improve outcomes after breast cancer treatment, it is essential to understand the mechanisms of action of potential therapeutic agents. The effect of fangchinoline (FAN) on migration and apoptosis of human breast cancer MDA-MB-231 cells and its underlying mechanisms were investigated. MDA-MB-231 cells were treated with different concentrations of FAN, growth inhibition rates were measured by MTT assay and morphological changes of apoptotic cells were observed by Hoechst staining. The wound-healing assay was used to determine of the effect of FAN on the migration of MDA-MB-231 cells. ELISA was used to detect the expression of MMP-2 and -9 in MDA-MB-231 cells treated with different concentrations of FAN and western blot analysis was used to quantify expression of NF-κβ and Iκβ proteins in the same cells. Our results showed that FAN significantly inhibited the growth of MDA-MB-231 cells in concentration-dependent manner and it induced MDA-MB-231 cell apoptosis. With the high FAN concentrations and long exposure times, the levels of MMP-2 and -9 decreased and the expression of NF-κβ decreased, while the expression of Iκβ protein increased. Based on these results, the antitumor effects of FAN on breast cancer cells can b...Continue Reading

Citations

Jun 8, 2019·International Journal of Oncology·Pantong LiYu Zhao
Oct 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Myriam MérarchiKwang Seok Ahn
Jul 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francis Yew Fu TiengAgustono Wibowo
Aug 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Young Yun JungKwang Seok Ahn
Sep 3, 2021·Biological & Pharmaceutical Bulletin·Yue LiJibin Li

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
flow cytometry
nuclear translocation

Software Mentioned

ImageJ
SPSS

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