FAP48, a new protein that forms specific complexes with both immunophilins FKBP59 and FKBP12. Prevention by the immunosuppressant drugs FK506 and rapamycin.

The Journal of Biological Chemistry
B ChambraudE E Baulieu

Abstract

We have identified a human gene encoding a 48-kDa protein that specifically interacts with the peptidyl prolyl isomerase FK506-binding protein 59 (FKBP59) and also with the well known FKBP12. FKBP59 and FKBP12 belong to the large family of immunophilins that bind the macrolide immunosuppressant drugs FK506 and rapamycin. The yeast two-hybrid system was used to isolate target proteins that interact with the immunosuppressant drug binding domain of the rabbit FKBP59. The cDNA for an as yet unidentified protein was isolated and cloned from a Jurkat cell library. The cDNA sequence of 1804 base pairs reveals an open reading frame of 417 amino acids. In vitro experiments suggest a direct interaction between FKBP59 and this new target protein. This specific association seems to be restricted to the FKBP family, since it also occurs both in vivo and in vitro with FKBP12 but not with cyclophilin 40. This novel protein was named FKBP-associated protein (FAP48). The formation of the complexes between FKBP59 or FKBP12 and FAP48 is prevented by FK506 and rapamycin in a dose-dependent manner. These results suggest that FAP48 shares or overlaps the macrolide binding site on FKBP59 as well as on FKBP12 and therefore may represent a natural com...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D A PeattieM Benasutti
Sep 30, 1992·Biochemical and Biophysical Research Communications·N MassolE E Baulieu
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·I CallebautJ P Mornon
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B E BiererS L Schreiber
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Jan 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·J M RenoirE E Baulieu
Sep 15, 1994·Biochemical and Biophysical Research Communications·M C LebeauC B Klee
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·C RadanyiE E Baulieu
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A M CameronS H Snyder
Jun 23, 1995·The Journal of Biological Chemistry·W M YangE Seto
Jan 1, 1995·Nature Medicine·S H Snyder, D M Sabatini
Apr 1, 1995·The Biochemical Journal·K Hoffmann, R E Handschumacher
Jan 1, 1993·Journal of Physiology, Paris·C Icard-LiepkalnsJ Mallet
Nov 24, 1994·Nature·M ThaliH G Göttlinger
Oct 15, 1993·Biochemical and Biophysical Research Communications·B ChambraudE E Baulieu
Sep 15, 1993·Biochemical and Biophysical Research Communications·S Le BihanE E Baulieu
Sep 15, 1993·European Journal of Biochemistry·A Galat
Dec 1, 1995·Trends in Biochemical Sciences·J B AllenS J Elledge
Aug 2, 1996·The Journal of Biological Chemistry·C S Hemenway, J Heitman

❮ Previous
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Citations

Dec 13, 1997·Molecular Neurobiology·D M SabatiniS H Snyder
Apr 1, 2004·Gene Expression Patterns : GEP·Brendan A S McIntyreMiikka Vikkula
Mar 24, 1998·Trends in Pharmacological Sciences·S H SnyderP D Suzdak
Sep 7, 2001·Trends in Plant Science·Y HarrarJ D Faure
Nov 10, 1998·The Plant Journal : for Cell and Molecular Biology·J D FaureS H Howell
Oct 21, 2003·DNA and Cell Biology·Nelly MassolEtienne-Emile Baulieu
Sep 9, 2004·BMC Pharmacology·Holger Neye, Eugen J Verspohl
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y MiyataE E Baulieu
Aug 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Takehiro AraiJames A DeCaprio
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·B ChambraudE E Baulieu
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ulrike KrummreiBéatrice Chambraud
Sep 23, 2014·Seminars in Pediatric Surgery·Ha-Long NguyenMiikka Vikkula
Mar 15, 2005·Critical Reviews in Biochemistry and Molecular Biology·Daniel L RiggsDavid F Smith
Apr 26, 2011·Current Opinion in Pharmacology·Jérôme SolassolThierry Maudelonde
Nov 26, 2010·Clinics in Plastic Surgery·Laurence M BoonMiikka Vikkula
May 20, 2003·Clinical Genetics·P Brouillard, M Vikkula
Jun 19, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tadashi NakagawaKeiichi I Nakayama
Jul 28, 2011·Pediatric Blood & Cancer·William E HaskinsElizabeth J Perlman
Jun 9, 2012·Journal of Cellular Biochemistry·Vincenzo EspositoAntonio Chirianni
Feb 15, 2002·American Journal of Human Genetics·Pascal BrouillardMiikka Vikkula
Sep 5, 1998·Neuron·S H SnyderP E Burnett
Dec 3, 2014·Immunology Letters·Pulak Ranjan Nath, Noah Isakov
Jan 28, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Ramtin RahbarEleanor N Fish
Mar 30, 2011·PloS One·Daqi TuMichael J Eck
Sep 18, 2012·PloS One·Abhinav KumarIan A Wilson
Jan 24, 2019·Biomolecules·Andreas HähleFelix Hausch
Apr 3, 2019·International Angiology : a Journal of the International Union of Angiology·Stefano PaolacciMatteo Bertelli
Oct 8, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Adahir Labrador-GarridoCintia Roodveldt

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