Far-infrared spectroscopy on free-standing protein films under defined temperature and hydration control

The Journal of Chemical Physics
Christian U StehleMartin Dressel

Abstract

The functionality of proteins is governed by their dynamics. We have performed a systematic investigation on four different proteins in the far-infrared spectral region under control of the two external parameters that have the strongest influence on the dynamics, namely temperature and hydration. The absorption measurements covering the frequency range from 40 cm(-1) to 690 cm(-1) (1-20 THz) close the gap between the well-studied mid-infrared and the recent THz investigations. By preparing the proteins as free-standing films, we achieve unprecedented reproducibility. Besides a featureless slope in the THz range, we can identify absorption peaks characteristic for each protein and others common to several proteins. We fit the spectra to extract the peak positions and suggest assignments for them. The far-infrared absorption spectra of all proteins are basically independent on hydration. By a detailed analysis of the sorption isotherms this can be explained by the low absorption of biological water, which resembles more the behavior of ice than that of liquid water.

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Citations

Aug 24, 2013·The Journal of Chemical Physics·Vladimir A Sirotkin, Aigul V Khadiullina
Dec 18, 2013·The Journal of Chemical Physics·Paola SassiAssunta Morresi
Mar 28, 2017·Journal of Biomedical Optics·Irina A BalakhninaIrina G Shpachenko
Jun 4, 2017·The Journal of Chemical Physics·Vladimir A Sirotkin, Alexandra A Kuchierskaya
Apr 20, 2018·Physical Chemistry Chemical Physics : PCCP·Yanchen HanXin Chen
Sep 19, 2020·Biotechnology for Biofuels·Yongni ShaoSonglin Zhuang
Oct 27, 2020·Journal of Chemical Information and Modeling·Tod D RomoAndrea G Markelz
Oct 11, 2017·The Journal of Physical Chemistry. B·Valeria Conti NibaliGiorgio Colombo

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