Far-red fluorescent protein excitable with red lasers for flow cytometry and superresolution STED nanoscopy.

Biophysical Journal
Kateryna S MorozovaV V Verkhusha

Abstract

Far-red fluorescent proteins are required for deep-tissue and whole-animal imaging and multicolor labeling in the red wavelength range, as well as probes excitable with standard red lasers in flow cytometry and fluorescence microscopy. Rapidly evolving superresolution microscopy based on the stimulated emission depletion approach also demands genetically encoded monomeric probes to tag intracellular proteins at the molecular level. Based on the monomeric mKate variant, we have developed a far-red TagRFP657 protein with excitation/emission maxima at 611/657 nm. TagRFP657 has several advantages over existing monomeric far-red proteins including higher photostability, better pH stability, lower residual green fluorescence, and greater efficiency of excitation with red lasers. The red-shifted excitation and emission spectra, as compared to other far-red proteins, allows utilizing TagRFP657 in flow cytometry and fluorescence microscopy simultaneously with orange or near-red fluorescence proteins. TagRFP657 is shown to be an efficient protein tag for the superresolution fluorescence imaging using a commercially available stimulated emission depletion microscope.

References

Jun 26, 2004·Chemistry & Biology·Vladislav V VerkhushaKonstantin A Lukyanov
Nov 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lei WangRoger Y Tsien
Jun 19, 2007·Nature Methods·Ekaterina M MerzlyakDmitriy M Chudakov
Aug 28, 2007·Nature Methods·Dmitry ShcherboDmitriy M Chudakov
Jan 1, 2009·Nature Methods·Stefan W Hell
Jan 16, 2009·The Biochemical Journal·Dmitry ShcherboDmitriy M Chudakov
Jan 1, 2009·Bioconjugate Chemistry·James A J FitzpatrickMarcel P Bruchez

❮ Previous
Next ❯

Citations

Jan 17, 2014·Journal of Chemical Biology·Jose Requejo-Isidro
May 9, 2012·Chemical Reviews·Fedor V Subach, Vladislav V Verkhusha
Jul 19, 2011·Nature Biotechnology·Grigory S FilonovVladislav V Verkhusha
Aug 2, 2011·Nature Methods·Oksana M SubachVladislav V Verkhusha
Dec 1, 2011·Nature Methods·Fedor V SubachVladislav V Verkhusha
Jun 19, 2013·Nature Methods·Daria M Shcherbakova, Vladislav V Verkhusha
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Roberto A ChicaStephen L Mayo
Sep 6, 2012·Acta Crystallographica. Section D, Biological Crystallography·Sergei PletnevVladimir Pletnev
Nov 13, 2012·PloS One·Olesya V StepanenkoKonstantin K Turoverov
Jun 20, 2014·Nature Reviews. Cancer·Marion de JongWytske M van Weerden
Mar 13, 2014·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Nikolaos C DeliolanisVasilis Ntziachristos
Jul 11, 2014·FEBS Letters·Tyler J ChozinskiJoshua C Vaughan
Jan 9, 2016·Journal of Molecular Biology·Karin Nienhaus, G Ulrich Nienhaus
Feb 4, 2014·Scientific Reports·Arie KrumholzVladislav V Verkhusha
Mar 20, 2013·Journal of Neurochemistry·Christian EggelingFrancisco J Barrantes
Nov 7, 2012·Biology of the Cell·Spencer C AlfordThomas Knöpfel
Jun 20, 2012·Journal of Biophotonics·Susan M Daly, Martin J Leahy
Mar 15, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sinem Saka, Silvio O Rizzoli
Jun 4, 2014·Protein Science : a Publication of the Protein Society·Timothy M Wannier, Stephen L Mayo
Jul 2, 2014·Journal of Internal Medicine·H Blom, H Brismar
Jun 27, 2015·Current Opinion in Chemical Biology·Daria M ShcherbakovaVladislav V Verkhusha
Jun 5, 2015·Current Opinion in Chemical Biology·Jhon Ralph EnterinaRobert E Campbell
May 14, 2014·Current Opinion in Chemical Biology·Daria M Shcherbakova, Vladislav V Verkhusha
Sep 25, 2012·Current Opinion in Structural Biology·Atsushi MiyawakiVladislav V Verkhusha
May 23, 2015·Quarterly Reviews of Biophysics·Christian EggelingStefan W Hell
Aug 6, 2011·Biochimica Et Biophysica Acta·Bogan S MelnikViktor P Kutyshenko
Feb 28, 2013·Biophysical Journal·Kevin T TakasakiBernardo L Sabatini
Jul 29, 2016·Current Opinion in Structural Biology·Ho-Leung Ng, Michael Z Lin
Jul 23, 2016·The Journal of Physical Chemistry Letters·Patrick E KonoldRalph Jimenez
Sep 21, 2016·Sensors·Bryce T BajarJun Chu
Dec 10, 2015·Scientific Reports·Anika HenseG Ulrich Nienhaus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved