Far Upstream Element-Binding Protein 1 Binds the 3' Untranslated Region of PKD2 and Suppresses Its Translation

Journal of the American Society of Nephrology : JASN
Wang ZhengXing-Zhen Chen

Abstract

Autosomal dominant polycystic kidney disease pathogenesis can be recapitulated in animal models by gene mutations in or dosage alterations of polycystic kidney disease 1 (PKD1) or PKD2, demonstrating that too much and too little PKD1/PKD2 are both pathogenic. Gene dosage manipulation has become an appealing approach by which to compensate for loss or gain of gene function, but the mechanisms controlling PKD2 expression remain incompletely characterized. In this study, using cultured mammalian cells and dual-luciferase assays, we found that the 3' untranslated region (3'UTR) of PKD2 mRNA inhibits luciferase protein expression. We then identified nucleotides 691-1044, which we called 3FI, as the 3'UTR fragment necessary for repressing the expression of luciferase or PKD2 in this system. Using a pull-down assay and mass spectrometry we identified far upstream element-binding protein 1 (FUBP1) as a 3FI-binding protein. In vitro overexpression of FUBP1 inhibited the expression of PKD2 protein but not mRNA. In embryonic zebrafish, FUBP1 knockdown (KD) by morpholino injection increased PKD2 expression and alleviated fish tail curling caused by morpholino-mediated KD of PKD2. Conversely, FUBP1 overexpression by mRNA injection significa...Continue Reading

References

Jul 1, 1992·Nature Genetics·S T Reeders
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·L TsiokasG Walz
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L TsiokasV P Sukhatme
Jan 11, 2000·Molecular and Cellular Biology·W WangM Gorospe
Jun 29, 2000·Annual Review of Biochemistry·A C GingrasN Sonenberg
Mar 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·S González-PerrettH F Cantiello
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Varani
Feb 14, 2002·Kidney International·Justin L RickerVincent H Gattone
Jan 9, 2004·The Journal of Biological Chemistry·Jin-Yu Lu, Robert J Schneider
Oct 22, 2004·Human Molecular Genetics·Irma S Lantinga-van LeeuwenDorien J M Peters
Mar 17, 2005·European Journal of Human Genetics : EJHG·Irma S Lantinga-van LeeuwenDorien J M Peters
Apr 22, 2005·Human Molecular Genetics·Qiang LiXing-Zhen Chen
Jun 23, 2005·Pflügers Archiv : European journal of physiology·Feng Qian, Konrad Noben-Trauth
Sep 2, 2005·Molecular and Cellular Biology·Rong MaLeonidas Tsiokas
Oct 11, 2005·Analytical Biochemistry·Mimi C Sammarco, Ed Grabczyk
Aug 15, 2008·The Journal of Biological Chemistry·Shuang FengAlbert C M Ong
Sep 11, 2008·The Journal of Biological Chemistry·Ingyu KimGuanqing Wu
Dec 23, 2008·The Journal of Biological Chemistry·Eun Young ParkJong Hoon Park
Jul 29, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Uta RabenhorstMartin Zörnig
Dec 25, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brandon W KusikAva J Udvadia
Jan 8, 2010·Human Molecular Genetics·Almira KurbegovicMarie Trudel
Jan 23, 2010·Nature Reviews. Molecular Cell Biology·Richard J JacksonTatyana V Pestova

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Citations

Jul 11, 2018·BMC Genomics·Stéphanie MoisanClaude Férec
Oct 22, 2018·Cellular and Molecular Life Sciences : CMLS·Lydie Debaize, Marie-Bérengère Troadec
Nov 5, 2021·Nature Reviews. Nephrology·Lisa SeufertRoman-Ulrich Müller

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