Far Upstream Element Binding Protein Plays a Crucial Role in Embryonic Development, Hematopoiesis, and Stabilizing Myc Expression Levels

The American Journal of Pathology
Weixin ZhouDavid Levens

Abstract

The transcription factor far upstream element binding protein (FBP) binds and activates the MYC promoter when far upstream element is via TFIIH helicase activity early in the transcription cycle. The fundamental biology and pathology of FBP are complex. In some tumors FBP seems pro-oncogenic, whereas in others it is a tumor suppressor. We generated an FBP knockout (Fubp1(-/-)) mouse to study FBP deficiency. FBP is embryo lethal from embryonic day 10.5 to birth. A spectrum of pathology is associated with FBP loss; besides cerebral hyperplasia and pulmonary hypoplasia, pale livers, hypoplastic spleen, thymus, and bone marrow, cardiac hypertrophy, placental distress, and small size were all indicative of anemia. Immunophenotyping of hematopoietic cells in wild-type versus knockout livers revealed irregular trilineage anemia, with deficits in colony formation. Despite normal numbers of hematopoietic stem cells, transplantation of Fubp1(-/-) hematopoietic stem cells into irradiated mice entirely failed to reconstitute hematopoiesis. In competitive transplantation assays against wild-type donor bone marrow, Fubp1(-/-) hematopoietic stem cells functioned only sporadically at a low level. Although cultures of wild-type mouse embryo fib...Continue Reading

References

Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y FujiwaraS H Orkin
Mar 5, 2002·Nature·Demetrios T BraddockG Marius Clore
Oct 27, 2004·Nature Structural & Molecular Biology·Fedor KouzineDavid Levens
Dec 17, 2004·Molecular and Cellular Biology·Achim WeberDavid Levens
Aug 18, 2006·Molecular and Cellular Biology·Hye-Jung ChungDavid Levens
Dec 7, 2007·The EMBO Journal·Gregg V CrichlowDemetrios Braddock
Jan 15, 2008·Nature Structural & Molecular Biology·Fedor KouzineDavid Levens
Nov 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lawrence R BenjaminDavid Levens
Dec 2, 2008·Cell Stem Cell·Elisa LaurentiAndreas Trumpp
Mar 7, 2009·Methods in Molecular Biology·Lino TessarolloVincenzo Coppola
Jul 9, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mona MalzKai Breuhahn
Jul 29, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Uta RabenhorstMartin Zörnig
Aug 17, 2010·Nature Structural & Molecular Biology·Cyprian D CukierAndres Ramos
Jul 23, 2011·Genes & Cancer·M Dolores Delgado, Javier León
Aug 6, 2011·Science·Chetan BettegowdaKenneth W Kinzler
Oct 2, 2012·Cell·Charles Y LinRichard A Young
Feb 19, 2013·Nature Structural & Molecular Biology·Fedor KouzineDavid Levens
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Huang LiYing Feng
Apr 3, 2014·Cold Spring Harbor Perspectives in Medicine·Laura A Johnston

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Citations

Oct 12, 2010·PloS One·Anna OnnisLorenzo Leoncini
Oct 7, 2016·International Journal of Biometeorology·Julia KälschHideo A Baba
Dec 25, 2019·Bioinformatics·Kristina L BuschurPanayiotis V Benos
Mar 17, 2017·Transcription·Leonie M Quinn
Oct 22, 2018·Cellular and Molecular Life Sciences : CMLS·Lydie Debaize, Marie-Bérengère Troadec
May 11, 2018·Journal of Virology·Jasmine Rae FrostPeter Pelka
Nov 4, 2020·International Journal of Molecular Sciences·Yam Prasad AryalJae-Young Kim

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