Farnesylation directs AtIPT3 subcellular localization and modulates cytokinin biosynthesis in Arabidopsis

Plant Physiology
Arnaud GalichetWilhelm Gruissem

Abstract

Cytokinins regulate cell division and differentiation as well as a number of other processes implicated in plant development. The first step of cytokinin biosynthesis in Arabidopsis (Arabidopsis thaliana) is catalyzed by adenosine phosphate-isopentenyltransferases (AtIPT). The enzymes are localized in plastids or the cytoplasm where they utilize the intermediate dimethylallyl-diphosphate from the methylerythritolphosphate or mevalonic acid pathways. However, the regulatory mechanisms linking AtIPT activity and cytokinin biosynthesis with cytokinin homeostasis and isoprenoid synthesis are not well understood. Here, we demonstrate that expression of AtIPT3, one member of the adenosine AtIPT protein family in Arabidopsis, increased the production of specific isopentenyl-type cytokinins. Moreover, AtIPT3 is a substrate of the protein farnesyl transferase, and AtIPT3 farnesylation directed the localization of the protein in the nucleus/cytoplasm, whereas the nonfarnesylated protein was located in the plastids. AtIPT3 gain-of-function mutant analysis indicated that the different subcellular localization of the farnesylated protein and the nonfarnesylated protein was closely correlated with either isopentenyl-type or zeatin-type cytok...Continue Reading

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Citations

Feb 16, 2011·Journal of Experimental Botany·Ivo FrébortPetr Galuszka
Dec 14, 2012·Molecular Plant-microbe Interactions : MPMI·Chang-Jie JiangHiroshi Takatsuji
Oct 31, 2012·International Journal of Molecular Sciences·Kirsten KrauseKarin Krupinska
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Apr 16, 2015·Journal of Experimental Botany·Anne Cortleven, Thomas Schmülling
May 28, 2019·Scientific Reports·Takatoshi KibaHitoshi Sakakibara
Oct 14, 2018·International Journal of Molecular Sciences·Vladimír SkalickýOndřej Novák
Jun 20, 2019·Frontiers in Plant Science·Chang-Jun LiuKewei Zhang
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Apr 14, 2019·Planta·Pavel JaworekPetr Tarkowski
Jun 26, 2020·Frontiers in Plant Science·Klára Hoyerová, Petr Hošek
May 1, 2012·Functional Plant Biology : FPB·Luk X Sp Chal

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