PMID: 7536048Feb 1, 1995Paper

Fas antigen-mediated apoptosis in human granulosa/luteal cells

Biology of Reproduction
S M QuirkK P Henrikson

Abstract

The Fas antigen is a transmembrane receptor that can trigger apoptosis in a variety of tumor and hematopoietic cells. Ovarian follicular atresia and luteolysis are thought to occur by apoptosis. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry, we demonstrated that human granulosa/luteal cells express the Fas antigen. An anti-human Fas antigen monoclonal antibody (Fas mAb; clone CH-11), which induces apoptosis in other cell types by binding to the Fas antigen, induced significant cell death (30%) in cultures pretreated with interferon gamma (IFN gamma). This agrees with studies on tumor cell lines showing that IFN gamma enhances cytotoxic effects of Fas mAb. Granulosa/luteal cells exhibited morphological characteristics typical of apoptosis, including membrane blebbing and condensed chromatin. DNA fragmentation into oligonucleosomal units of approximately 180 bp, typical of apoptosis, was detected at elevated levels in Fas mAb-treated cultures via 3' end-labeling and gel electrophoresis. Examination of cultured cells in situ for apoptotic DNA cleavage by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end-labeling (TUNEL) indicated that more apoptotic death occurred in...Continue Reading

Citations

Nov 12, 1998·The Anatomical Record·Y FukazawaY Ohta
May 1, 1997·Molecular Reproduction and Development·K SakamakiS Yonehara
Jul 16, 2008·Apoptosis : an International Journal on Programmed Cell Death·Dmitri V KryskoKatharina D'Herde
May 1, 1996·Theriogenology·W Hansel, G Cain
Aug 1, 1996·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D VinatierD Subtil
Jun 19, 2002·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Christophe SiferGerard Feldmann
Aug 23, 2003·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Christophe SiferJean-Louis Benifla
Sep 21, 2002·Molecular and Cellular Endocrinology·Tommi E VaskivuoJuha S Tapanainen
Jul 1, 1997·Trends in Endocrinology and Metabolism : TEM·S Y Hsu, A J Hsueh
Jun 12, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C V Andreu-Vieyra, H R Habibi
Jun 1, 2002·Ageing Research Reviews·Peter J Hornsby
May 1, 1998·Zygote : the Biology of Gametes and Early Embryos·J P XuT Mori
May 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Luiz E HenkesBo R Rueda
Sep 1, 1996·The Journal of Experimental Medicine·J O'ConnellF Shanahan
Sep 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·K V Konan, M W Taylor
Feb 27, 2004·Biology of Reproduction·Marissa L HammDavid R Mann
May 1, 1996·Obstetrical & Gynecological Survey·S J SpencerR B Jaffe
May 1, 1997·American Journal of Reproductive Immunology : AJRI·M W GuoT Mori
Jan 6, 2000·American Journal of Reproductive Immunology : AJRI·J P XuT Mori
Jan 1, 1997·Annual Review of Physiology·A Kaipia, A J Hsueh
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Luiz E HenkesBo R Rueda
Mar 26, 2003·Cell Structure and Function·Kazuhiro Sakamaki
Dec 26, 2009·The Journal of Reproduction and Development·Takuo HojoKiyoshi Okuda
Jun 4, 2010·The Journal of Reproduction and Development·Miki SugimotoNoboru Manabe
May 10, 2011·The Journal of Reproduction and Development·Naoko InoueNoboru Manabe
Aug 24, 2006·The Journal of Reproduction and Development·Naoko InoueNoboru Manabe
Aug 24, 2006·The Journal of Reproduction and Development·Fuko Matsuda-MinehataNoboru Manabe
Dec 21, 2006·The Journal of Reproduction and Development·Naoko InoueNoboru Manabe
Nov 7, 2007·The Journal of Reproduction and Development·Norihiro Sugino, Kiyoshi Okuda
Jul 8, 2008·The Journal of Reproduction and Development·Fuko MatsudaNoboru Manabe
Mar 28, 2012·The Journal of Reproduction and Development·Fuko MatsudaSatoshi Ohkura
Mar 31, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Takashi ShimizuYukio Kanai
Jun 13, 2013·Theriogenology·H-J LiB Shorgan
Mar 29, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Sandra S VallcanerasAna M Rastrilla
Mar 11, 2003·Reproductive Biomedicine Online·Tommi E Vaskivuo, Juha S Tapanainen
Jul 24, 2010·Molecular Reproduction and Development·Pengfei Lin, Rong Rui
Mar 26, 2002·Biochemical Pharmacology·Seong Gon KimKenneth A Jacobson
Jun 17, 2006·Gynécologie, obstétrique & fertilité·G FeldmannP Madelenat
May 18, 1999·Gynecologic Oncology·H J Garbán, B Bonavida
Feb 18, 2017·International Journal of Molecular Sciences·Tesfaye WorkuLiguo Yang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.