Fas signalling promotes intercellular communication in T cells.

PloS One
Francesca LuchettiStefano Papa

Abstract

Cell-to-cell communication is a fundamental process for development and maintenance of multicellular organisms. Diverse mechanisms for the exchange of molecular information between cells have been documented, such as the exchange of membrane fragments (trogocytosis), formation of tunneling nanotubes (TNTs) and release of microvesicles (MVs). In this study we assign to Fas signalling a pivotal role for intercellular communication in CD4+ T cells. Binding of membrane-bound FasL to Fas expressing target cells triggers a well-characterized pro-apoptotic signalling cascade. However, our results, pairing up flow cytometric studies with confocal microscopy data, highlight a new social dimension for Fas/FasL interactions between CD4+ T cells. Indeed, FasL enhances the formation of cell conjugates (8 fold of increase) in an early time-frame of stimulation (30 min), and this phenomenon appears to be a crucial step to prime intercellular communication. Our findings show that this communication mainly proceeds along a cytosolic material exchange (ratio of exchange >10, calculated as ratio of stimulated cells signal divided by that recorded in control cells) via TNTs and MVs release. In particular, inhibition of TNTs genesis by pharmacologi...Continue Reading

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Dec 12, 2012·Cell and Tissue Research·Shunsuke KimuraHiroshi Ohno
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Jul 2, 2021·Frontiers in Immunology·Vaibhav TiwariDeepak Shukla

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Methods Mentioned

BETA
flow cytometry
confocal microscopy
flow
FCS

Software Mentioned

Adobe Photoshop
CellQuest
ImageJ

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